Carbon nanotube-modified carbon fiber microelectrodes for in vivo voltammetric measurement of ascorbic acid in rat brain.
Carbon nanotube-modified carbon fiber microelectrodes for in vivo voltammetric measurement of ascorbic acid in rat brain.
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DOI：
10.1021/ac0705871
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发表时间：
2007-08
影响因子：
7.4
通讯作者：
Meining Zhang;Kun Liu;Ling Xiang;Yuqing Lin;L. Su;L. Mao
中科院分区：
文献类型：
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作者：
Meining Zhang;Kun Liu;Ling Xiang;Yuqing Lin;L. Su;L. Mao
基于多壁碳纳米管(MWNT)对抗坏血酸(AA)氧化的电化学性质，提出了一种利用多壁碳纳米管(MWNT)修饰的碳纤维微电极(CFME)对大鼠脑组织中抗坏血酸(AA)进行体内检测的新方法。循环伏安结果表明，所制备的多壁碳纳米管修饰的CFME对AA氧化具有明显的电催化活性，可用于3，4-二羟基苯乙酸、尿酸、5-羟色胺等多种电活性物质在大鼠脑内共存时的选择性测定。通过将抗坏血酸氧化酶外源性注入脑内，进一步研究了修饰的碳纳米管对AA测量的选择性，结果证实，所制备的电极具有选择性，因此可以用于可靠的大鼠脑内AA的在体测量，并且在体内测量中具有良好的稳定性。纹状体AA的基础水平为0.20±0.05 mm(n=3)。初步证明了多壁碳纳米管修饰的CFME伏安法可用于观察脑内外源性AA对纹状体AA的动态平衡调节。
This study demonstrates a new electrochemical method for in vivo measurements of ascorbic acid (AA) in rat brain with multiwalled carbon nanotube (MWNT)-modified carbon fiber microelectrodes (CFMEs) based on the electrochemical property of MWNTs for facilitating AA oxidation. Cyclic voltammetry results indicate that the prepared MWNT-modified CFMEs possess a marked electrocatalytic activity toward AA oxidation and can be used for its selective measurement in the presence of other kinds of electroactive species coexisting in rat brain, such as 3,4-dihydroxyphenylacetic acid, uric acid, and 5-hydroxytryptamine. The selectivity of the MWNT-modified CFMEs toward AA measurement is further studied in vivo by exogenously infusing ascorbate oxidase into the brain, and the results confirm that the prepared electrodes are selective and can thus be used for reliable in vivo measurements of AA in rat brain, combined with their good stability during in vivo measurements. The basal level of striatum AA is determined to be 0.20 +/- 0.05 mM (n = 3). The application of the voltammetric method with the MWNT-modified CFMEs is preliminarily demonstrated for in vivo observation of homeostatic regulation of striatum AA with exogenous infusion of AA into the brain.