Granulocyte-macrophage colony-stimulating factor activates macrophages derived from bone marrow cultures to synthesis of MHC class II molecules and to augmented antigen presentation function.
Granulocyte-macrophage colony-stimulating factor activates macrophages derived from bone marrow cultures to synthesis of MHC class II molecules and to augmented antigen presentation function.
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粒细胞-巨噬细胞集落刺激因子激活来自骨髓培养物的巨噬细胞合成 MHC II 类分子并增强抗原呈递功能。
DOI：
10.4049/jimmunol.141.11.3882
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发表时间：
1988
影响因子：
4.4
通讯作者：
A. Reske
中科院分区：
文献类型：
--
作者：
Hans;S. Frosch;K. Reske;A. Reske
本文观察了粒细胞-巨噬细胞（GM）-CSF对骨髓源性巨噬细胞（BMM phi）合成MHC Ⅱ类分子和提呈抗原能力的影响。巨噬细胞-CSF体外培养获得的BMM phi不合成I-A分子，诱导Ag介导的胰岛素特异性T克隆细胞增殖的效率低于脾辅助细胞。经GM-CSF脉冲处理24 ~ 48 h后，第12天BMM phi呈现出高效的抗原提呈功能，其提呈功能上级优于IFN-γ。膜结合IL-1的表达被GM-CSF显著增强，但不被IFN-γ增强。然而，用于探测BMM phi的辅助细胞功能的T细胞克隆不依赖于IL-1以实现最佳增殖。同时，GM-CSF诱导I-A分子的从头合成，尽管程度低于最佳剂量的IFN-γ。因此，GM-CSF似乎引发除了Ia分子合成和BMM phi中的膜IL-1表达之外的性质，所述BMM phi中的膜IL-1表达对于T克隆细胞的有效辅助细胞功能是必需的。GM-CSF对BMM phi的激活作用是可逆的，并且可以重复。这些数据表明，GM-CSF对预先形成的BMM phi发挥调节作用，可逆地激活细胞的Ia生物合成潜力和明显的辅助细胞能力，从而使前体细胞分化为组成性功能状态的解释不太可能发生。
The effects of granulocyte-macrophage (GM)-CSF on the synthesis of MHC class II molecules and on the Ag presentation capacity by bone marrow derived macrophages (BMM phi) was investigated. BMM phi obtained by in vitro culture in the presence of macrophage-CSF were negative for synthesis of I-A molecules and induced the Ag-mediated proliferation of insulin-specific T clone cells with lower efficiency than splenic accessory cells. After pulse treatment with GM-CSF for 24 to 48 h, day 12 BMM phi exhibited highly efficient Ag presentation function which was superior to that induced by IFN-gamma. Expression of membrane-bound IL-1 was augmented significantly by GM-CSF, but not by IFN-gamma. However, the T cell clone used to probe for accessory cell function of BMM phi was not dependent on IL-1 for optimal proliferation. Concomitantly, GM-CSF induced the de novo synthesis of I-A molecules, although to a lesser extent than optimal doses of IFN-gamma. Thus GM-CSF appears to elicit properties in addition to Ia molecule synthesis and membrane IL-1 expression in BMM phi being essential for efficient accessory cell function to the T clone cells. The activation of BMM phi by GM-CSF was reversible and could be repeated. These data show that GM-CSF exerts a modulatory influence on preformed BMM phi, reversibly activating cells to Ia biosynthetic potential and pronounced accessory cell capacity, thus rendering the explanation unlikely that differentiation of precursor cells into a constitutively functional state had occurred.