Translocation of Intratracheally Instilled Multiwall Carbon Nanotubes to Lung-Associated Lymph Nodes in Rats
Translocation of Intratracheally Instilled Multiwall Carbon Nanotubes to Lung-Associated Lymph Nodes in Rats
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DOI：
10.2486/indhealth.ms1213
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发表时间：
2011-03-01
影响因子：
2
通讯作者：
Fukushima, Shoji
中科院分区：
文献类型：
--
作者：
Aiso, Shigetoshi;Kubota, Hisayo;Fukushima, Shoji
为了评估多壁碳纳米管（MWCNT）的肺外效应，在给予MWCNT的大鼠中检查了MWCNT的沉积和肺相关淋巴结（LALN）的组织病理学变化。在13周龄时，向雄性F344大鼠气管内滴注剂量为0的MWCNT（溶媒）。40或160 μ g/大鼠。在第1天处死大鼠。分别于滴注后7、28或91小时取兔膝关节韧带组织行光镜检查。多壁碳纳米管移位至右侧和左侧后纵隔淋巴结和甲状旁腺淋巴结。多壁碳纳米管沉积在后纵隔淋巴结大于甲状旁腺淋巴结，沉积在这两个淋巴结的多壁碳纳米管的量随着时间的推移逐渐增加，并呈剂量依赖性。多壁碳纳米管被淋巴结巨噬细胞吞噬，一些载有多壁碳纳米管的巨噬细胞聚集。透射电子显微镜（TEM）观察证实了多壁碳纳米管纤维的特征多壁圆柱形结构的存在。
In order to assess the extrapulmonary effects of multiwall carbon nanotubes (MWCNT), deposition of MWCNT and histopathologic changes in lung-associated lymph nodes (LALN) were examined in MWCNT-administered rats. At the age of 13 wk, male F344 rats were intratracheally instilled with MWCNT at a dose of 0 (vehicle). 40 or 160 mu g/rat. The rats were sacrificed on Day I. 7, 28 or 91 after instillation and light microscopic examinations were performed on LALN tissues. MWCNT was translocated to right and left posterior mediastinal lymph nodes and parathymic lymph nodes. Deposition of MWCNT was greater in the posterior mediastinal lymph node than in the parathymic lymph node, and the amount of MWCNT deposited in these two lymph nodes increased gradually and dose-dependently with time. MWCNT was phagocytosed by nodal macrophages, and some of the MWCNT-laden macrophages were aggregated. Transmission electron microscopic (TEM) observation confirmed the presence of MWCNT fibers with a characteristic multi-walled cylindrical structure.