TOXICITY AND PARTIAL STRUCTURE OF A HEPATOTOXIC PEPTIDE PRODUCED BY THE CYANOBACTERIUM NODULARIA-SPUMIGENA MERTENS EMEND L575 FROM NEW-ZEALAND
TOXICITY AND PARTIAL STRUCTURE OF A HEPATOTOXIC PEPTIDE PRODUCED BY THE CYANOBACTERIUM NODULARIA-SPUMIGENA MERTENS EMEND L575 FROM NEW-ZEALAND
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DOI：
10.1128/aem.54.9.2257-2263.1988
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发表时间：
1988-09-01
影响因子：
4.4
通讯作者：
MOORE, RE
中科院分区：
文献类型：
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作者：
CARMICHAEL, WW;ESCHEDOR, JT;MOORE, RE
丝状微咸水蓝藻的一个克隆分离物，命名为L575。被发现产生一种有效的肝毒性多肽(对小鼠的50%致死剂量，60微克/公斤)，其化学和毒理学特性与其他淡水浮游蓝藻产生的肝毒性七肽相似。该分离物是从1980年在新西兰埃尔斯米尔湖采集的水样中提取的。该毒素经高效液相色谱分离纯化和氨基酸分析，含有等量的谷氨酸、β-甲基天冬氨酸和精氨酸单元。该毒素的快原子轰击质谱图显示其相对分子质量为824。菌株L575的分批培养表明，毒素含量在1.96~2.99 mg/g细胞之间变化，且毒素含量与叶绿素a呈正相关，而与生物量无关。
A clonal isolate, termed L575, of the filamentous brackish-water cyanobacterium Nodularia spumigena Mertens emend. was found to produce a potent hepatotoxic peptide (50% lethal intraperitoneal dose for the mouse, 60 .mu.g/kg) with chemical and toxicological properties similar to those of the hepatotoxic heptapeptides produced by other freshwater planktonic cyanobacteria. The isolate was made from a water sample collected in Lake Ellesmere, New Zealand, in 1980. The toxin, isolated and purified by high-performance liquid chromatography (HPLC) and analyzed by HPLC amino acid anaysis, contained glutamic acid, .beta.-methylaspartic acid, and arginine units in equivalent amounts. The fast-atom-bombardment mass spectrum of the toxin indicated the molecular weight to be 824. Batch cultures of strain L575 showed that the toxin content varied between 1.96 and 2.99 mg/g of cells and that a positive correlation between toxin content and chlorophyll a, but not biomass, was present.