Molecular phylogenetic analysis of Fasciola flukes from eastern India
Molecular phylogenetic analysis of Fasciola flukes from eastern India
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DOI：
10.1016/j.parint.2015.04.004
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发表时间：
2015-10-01
影响因子：
1.9
通讯作者：
Itagaki, Tadashi
中科院分区：
文献类型：
--
作者：
Hayashi, Kei;Ichikawa-Seki, Madoka;Itagaki, Tadashi
来自印度东部的片形吸虫的特点是精子发生状态和核ITS1的基础上。在Imphal、科希马和Gantoku区均检测到片形吸虫和无精子片形吸虫。线粒体nad1基因序列分析，推测其与周边国家的亲缘关系。无精子片形吸虫的单倍型与邻国人群的单倍型相同或显示出单核苷酸取代，证实了先前将它们归类为同一谱系的报道。然而，在印度东部的无精子片形吸虫的患病率低于邻国，这表明他们还没有分散在整个印度东部。与此相反，F.南极吸虫是主要的和多样化的，该物种被认为是在该地区分布的时间比无精子片形吸虫。来自印度东部的片形吸虫种群被分为两个不同的单倍群A和B。它们的遗传多样性水平表明，属于单倍群A的人群已经从印度次大陆的西侧分散到印度东部的人工移动的国内牛，牛indicus，而属于单倍群B的人群可能已经从缅甸传播到印度东部的国内水牛，水牛。(C)2015爱思唯尔爱尔兰有限公司版权所有。
Fasciola flukes from eastern India were characterized on the basis of spermatogenesis status and nuclear ITS1. Both Fasciola gigantica and aspermic Fasciola flukes were detected in Imphal, Kohima, and Gantoku districts. The sequences of mitochondrial nad1 were analyzed to infer their phylogenetical relationship with neighboring countries. The haplotypes of aspermic Fasciola flukes were identical or showed a single nucleotide substitution compared to those from populations in the neighboring countries, corroborating the previous reports that categorized them in the same lineage. However, the prevalence of aspermic Fasciola flukes in eastern India was lower than those in the neighboring countries, suggesting that they have not dispersed throughout eastern India. In contrast, F. gigantica was predominant and well diversified, and the species was thought to be distributed in the area for a longer time than the aspermic Fasciola flukes. Fasciola gigantica populations from eastern India were categorized into two distinct haplogroups A and B. The level of their genetic diversity suggests that populations belonging to haplogroup A have dispersed from the west side of the Indian subcontinent to eastern India with the artificial movement of domestic cattle, Bos indicus, whereas populations belonging to haplogroup B might have spread from Myanmar to eastern India with domestic buffaloes, Bubalus bubalis. (C) 2015 Elsevier Ireland Ltd. All rights reserved.