A comprehensive genetic map of murine chromosome 11 reveals extensive linkage conservation between mouse and human.
A comprehensive genetic map of murine chromosome 11 reveals extensive linkage conservation between mouse and human.
复制标题
小鼠 11 号染色体的综合遗传图谱揭示了小鼠和人类之间广泛的连锁保守性。
DOI：
10.1093/genetics/122.1.153
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发表时间：
1989
期刊：
影响因子：
3.3
通讯作者：
Copeland,NG
中科院分区：
文献类型：
--
作者：
Buchberg,AM;Brownell,E;Nagata,S;Jenkins,NA;Copeland,NG
利用C57BL/6J与大鼠杂交的种间回交动物，建立了小鼠11号染色体的综合连锁图谱。通过体细胞杂交或回交分析确定先前分配到小鼠11号染色体上的基因（Erbb、Rel、11-3、Csfgm、Trp53-1、Evi-2、Erba、Erbb-2、Csfg、Myhs、Cola-1、Myla、Hox-2和Pkca）的相对定位。我们还分析了我们怀疑可能映射到11号染色体的基因，因为它们在人类染色体中的位置，以及小鼠11号染色体与人类染色体之间已知的连锁同源性（Mpo, Ngfr， Pdgfr和Fms）。后者的两个基因，Mpo和Ngfr，被定位到小鼠的11号染色体上。这两个基因也被定位到人类17号染色体上，扩展了人类17号染色体和小鼠11号染色体之间的连锁保护程度。Pdgfr和Fms与人类5号染色体上的II-3和Csfgm密切相关，它们被定位到小鼠18号染色体上，而不是小鼠11号染色体上，从而定义了人类和小鼠染色体之间的另一个保守连锁群。这些研究中生成的小鼠11号染色体连锁图谱极大地扩展了识别小鼠中涉及人类疾病的同源基因的框架，阐明了导致几种小鼠突变的基因，并获得了对染色体进化和基因组组织的见解。
Interspecific backcross animals from a cross between C57BL/6J and Mus spretus mice were used to generate a comprehensive linkage map of mouse chromosome 11. The relative map positions of genes previously assigned to mouse chromosome 11 by somatic cell hybrid or genetic backcross analysis were determined (Erbb, Rel, 11-3, Csfgm, Trp53-1, Evi-2, Erba, Erbb-2, Csfg, Myhs, Cola-1, Myla, Hox-2 and Pkca). We also analyzed genes that we suspected would map to chromosome 11 by virtue of their location in human chromosomes and the known linkage homologies that exist between murine chromosome 11 and human chromosomes (Mpo, Ngfr, Pdgfr and Fms). Two of the latter genes, Mpo and Ngfr, mapped to mouse chromosome 11. Both genes also mapped to human chromosome 17, extending the degree of linkage conservation observed between human chromosome 17 and mouse chromosome 11. Pdgfr and Fms, which are closely linked to II-3 and Csfgm in humans on chromosome 5, mapped to mouse chromosome 18 rather than mouse chromosome 11, thereby defining yet another conserved linkage group between human and mouse chromosomes. The mouse chromosome 11 linkage map generated in these studies substantially extends the framework for identifying homologous genes in the mouse that are involved in human disease, for elucidating the genes responsible for several mouse mutations, and for gaining insights into chromosome evolution and genome organization.