CrAssphage as a Potential Human Sewage Marker for Microbial Source Tracking in Southeast Asia
CrAssphage as a Potential Human Sewage Marker for Microbial Source Tracking in Southeast Asia
复制标题
DOI：
10.1021/acs.estlett.9b00041
复制
发表时间：
2019-03-01
影响因子：
10.9
通讯作者：
Sirikanchana, Kwanrawee
中科院分区：
文献类型：
--
作者：
Kongprajug, Akechai;Mongkolsuk, Skorn;Sirikanchana, Kwanrawee
人类肠道噬菌体 crAssphage 已被提议作为受影响水体的人类特异性微生物源追踪 (MST) 标记。然而，其作为人类特异性 MST 标记的全球使用需要在热带地区进行验证。本研究在泰国 21 个污水样本中检测到了 crAssphage qPCR 标记物 (CPQ_056)，灵敏度为 100%。在医院和住宅楼的污水中检测到该标记物，浓度为 5.28-7.38 log(10) 拷贝/100 mL，在城市污水处理厂的四个进水和四个出水样品中检测到的标记物浓度分别为 4.23-6.19 和 3.78-4.89 log(10) 拷贝/100 mL。此外，使用猪、牛、鸡、鸭、山羊、绵羊、水牛和鱼的粪便观察到 99.2% 的特异性 (n = 127)，仅在一份复合猪样本中发生交叉检测。 crAssphage 标记物存在于 56.25%（48 个中的 27 个）的河流样本中，含量为 3.20-7.29 log(10) 拷贝/100 mL。 crAssphage 标记物和预先验证的人类特异性拟杆菌标记物 (HF183/BFDrev) 的浓度在任何污水或废水样品中都没有显着差异，而 crAssphage 标记物丰度在河流样品中更高。 crAssphage 基因作为东南亚人类特异性 MST 标记的初步验证将促进其纳入 MST 工具箱。
The human gut bacteriophage crAssphage has been proposed as a human-specific microbial source tracking (MST) marker for impacted water bodies. However, its global use as a human-specific MST marker requires validation in a tropical region. In this study, a crAssphage qPCR marker (CPQ_056) was detected in 21 sewage samples in Thailand with 100% sensitivity. The marker was detected in sewage from hospitals and residential buildings at 5.28-7.38 log(10) copies/100 mL and in four influent and four effluent samples of municipal wastewater treatment plants at 4.23-6.19 and 3.78-4.89 log(10) copies/100 mL, respectively. Furthermore, a 99.2% specificity (n = 127) was observed using feces from swine, cattle, chicken, duck, goat, sheep, buffalo, and fish, with cross-detection only occurring for one composite swine sample. The crAssphage marker was present in 56.25% (27 out of 48) of river samples at 3.20-7.29 log(10) copies/100 mL. The concentrations of the crAssphage marker and a prevalidated human-specific Bacteroidales marker (HF183/BFDrev) did not differ significantly in any of the sewage or wastewater samples, whereas the crAssphage marker abundance was higher in river samples. This initial validation of the crAssphage gene as a human-specific MST marker in Southeast Asia will promote its inclusion in an MST toolbox.