Adrenal, pituitary and urinary ascorbic acid levels in rats subjected to hypothermic environment.
Adrenal, pituitary and urinary ascorbic acid levels in rats subjected to hypothermic environment.
复制标题
低温环境下大鼠的肾上腺、垂体和尿抗坏血酸水平。
DOI：
10.1152/ajplegacy.1954.178.3.457
复制
发表时间：
1954
期刊：
影响因子：
--
通讯作者：
P. Osher
中科院分区：
文献类型：
--
作者：
S. Katsh;G. F. Katsh;P. Osher
材料和方法：将总共67只成年雄性白化病大鼠（Sprague-Dawley）饲养在单独的代谢笼中，代谢笼置于大玻璃漏斗上，漏斗通向用于收集尿液的量筒。通过将蒸发皿倒置放置在大玻璃漏斗中实现尿液与粪便的分离，作为额外的预防措施，将含有玻璃棉的小玻璃漏斗置于大漏斗和圆筒之间。笼具设有通向外部杯的食物通道。可自由进食不含可测量量抗坏血酸的食物2和水。隔日测量摄食量和饮水量以及尿量和体重，但进行分析时除外，此时每天进行测量。将29只大鼠分为5组（IV），
MATERIALS AND METHODSA total of 67 adult male albino rats (Sprague-Dawley) were housed in individual metabolism cages which rested upon large glass funnels leading to graduated cylinders for the collection of urine. Separation of urine from feces was accomplished by placing an evaporating dish in an inverted position in the large glass funnels and as an added precaution small glass funnels containing glass wool were interposed between the large funnels and the cylinders. The cages were provided with food channels leading to outside cups. Food, 2 which contained no measurable amount of ascorbic acid, and water were available ad libitum. Measurements of food and water consumption as well as urine volume and body weights were made on alternate days except when analyses were performed at which time daily measurements were made. Twentynine rats were dispersed into five groups (IV) and