Expression and differential posttranscriptional regulation of the <i>elongation factor 1 alpha 1</i> gene in endometrial caruncle and intercaruncle of Japanese Black cattle at early and mid‐gestation stages
Expression and differential posttranscriptional regulation of the <i>elongation factor 1 alpha 1</i> gene in endometrial caruncle and intercaruncle of Japanese Black cattle at early and mid‐gestation stages
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日本黑牛妊娠早中期子宫内膜肉阜和肉阜间<i>elongation Factor 1 α1</i>基因的表达及差异转录后调控
DOI：
10.1111/asj.13746
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发表时间：
2022
影响因子：
2
通讯作者：
Oshima Kazunaga
中科院分区：
文献类型：
--
作者：
Komatsu Masanori;Mannen Hideyuki;Taniguchi Masaaki;Oshima Kazunaga
延伸因子1 α 1 （EEF1A1）是EEF1A的异构体，是最丰富的细胞质蛋白之一，也是翻译机制的重要组成部分。我们研究了feef1a1mrna在日本黑牛妊娠早期和中期子宫内膜caruncle （CAR）和intercaruncle （ICAR）中的相对表达、选择性聚腺苷化（APA）和聚(A)尾长变化。CAR中eef1a1mrna的相对表达水平在妊娠第20天最高，在妊娠中期显著降低。ICAR的表达水平明显高于CAR，而妊娠期对其无显著影响。鉴定出4种不同的teef1a1转录本，它们具有不同的3 '非翻译区（UTRs）（近端和远端类型）和不同长度的多(A)尾部（中短类型）。具有远端3 ' UTR和中长度多尾(A)的eef1a1mrna是妊娠早期子宫角CAR特异性的。RNA测序数据分析显示，hsf1、MZF1、E47、SRF、GATA2、GATA3、GATA6、HNF‐3 β (FOXA2)、CPSF1和ataxin‐2基因可能影响eef1a1基因的表达或poly(A)长度。
The elongation factor 1 alpha 1 (EEF1A1), an isoform of EEF1A, is one of the most abundant cytoplasmic proteins and an important component of the translational machinery. We investigated the relative expression, alternative polyadenylation (APA), and changes in poly(A) tail length ofEEF1A1mRNA in the endometrial caruncle (CAR) and intercaruncle (ICAR) at early and mid‐gestation in Japanese Black cattle. The relativeEEF1A1mRNA expression levels in the CAR were the highest on Gestation day 20 and were significantly decreased at mid‐gestation. The expression levels in the ICAR were significantly higher than those in the CAR, and the gestation stage had no significant impact. Four differentEEF1A1transcripts with distinct 3′ untranslated regions (UTRs) (proximal and distal types) and poly(A) tails (medium and short types) of different lengths were identified. TheEEF1A1mRNAs with distal 3′ UTR and medium‐length poly(A) tails were specific from the CAR of uterus horn at early gestation. RNA‐sequencing data analyses revealed that theHSF1,MZF1,E47,SRF,GATA2,GATA3,GATA6,HNF‐3 beta(FOXA2),CPSF1, andAtaxin‐2genes might affect theEEF1A1gene expression or poly(A) length.