Role of polyisoprenoids in tobacco resistance against biotic stresses
Role of polyisoprenoids in tobacco resistance against biotic stresses
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DOI：
10.1111/j.1399-3054.2009.01204.x
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发表时间：
2009-04-01
影响因子：
6.4
通讯作者：
Swiezewska, Ewa
中科院分区：
文献类型：
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作者：
Bajda, Agnieszka;Konopka-Postupolska, Dorota;Swiezewska, Ewa
无毒病原体、烟草花叶病毒 (TMV) 或丁香假单胞菌感染。烟粉虱诱导抗性烟草植物烟草 (Nicotiana tabacum cv.) 叶片中聚异戊二烯醇、茄尼醇和聚异戊二烯醇家族的积累 [从由 14 个异戊二烯单元 (Pren-14) 到 -18 组成的聚异戊二烯单元 (Pren-16 占主导地位)] 积累。萨姆松 NN。 TMV感染后，感染后第7天，接种叶和上部叶中的茄尼醇含量分别增加了7倍和8倍，而接种叶和上部叶中的聚戊烯醇含量分别增加了2.5倍和2倍。外源施加的过氧化氢也刺激聚异戊二烯醇的积累，但外源水杨酸（SA）则不刺激。相反，接种易感烟草植物的叶子或伤害烟草叶子都不会导致类聚异戊二烯含量增加。综上所述，这些结果表明聚异戊二烯醇可能参与植物对病原体的抵抗力。讨论了积累的聚异戊二烯在植物对病原体攻击的反应中的假定作用。同样，接种 TMV 的抗性植物上部叶片中的质体醌 (PQ) 含量增加了两倍。过氧化氢、细菌（丁香假单胞菌）和 SA 也会刺激 PQ 的积累。 PQ 在细胞膜区室抗氧化防御中的作用在受病毒感染的烟叶中激活的酶抗氧化机制的背景下进行了讨论。在抗性烟草植物的接种叶片中发现几种抗氧化酶（抗坏血酸过氧化物酶、愈创木酚过氧化物酶、谷胱甘肽还原酶和超氧化物歧化酶，特别是铜锌超氧化物歧化酶亚型）活性升高，过氧化氢酶短暂但短暂升高，但在抗性烟草植物中未发现，但在易感植物中却没有。
Infection with avirulent pathogens, tobacco mosaic virus (TMV) or Pseudomonas syringae pv. tabaci induced accumulation of polyisoprenoid alcohols, solanesol and a family of polyprenols [from polyprenol composed of 14 isoprene units (Pren-14) to -18, with Pren-16 dominating] in the leaves of resistant tobacco plants Nicotiana tabacum cv. Samsun NN. Upon TMV infection, solanesol content was increased seven- and eight-fold in the inoculated and upper leaves, respectively, while polyprenol content was increased 2.5- and 2-fold in the inoculated and upper leaves, respectively, on the seventh day post-infection. Accumulation of polyisoprenoid alcohols was also stimulated by exogenously applied hydrogen peroxide but not by exogenous salicylic acid (SA). On the contrary, neither inoculation of the leaves of susceptible tobacco plants nor wounding of tobacco leaves caused an increase in polyisoprenoid content. Taken together, these results indicate that polyisoprenoid alcohols might be involved in plant resistance against pathogens. A putative role of accumulated polyisoprenoids in plant response to pathogen attack is discussed. Similarly, the content of plastoquinone (PQ) was increased two-fold in TMV-inoculated and upper leaves of resistant plants. Accumulation of PQ was also stimulated by hydrogen peroxide, bacteria (P. syringae) and SA. The role of PQ in antioxidant defense in cellular membranous compartments is discussed in the context of the enzymatic antioxidant machinery activated in tobacco leaves subjected to viral infection. Elevated activity of several antioxidant enzymes (ascorbate peroxidase, guaiacol peroxidase, glutathione reductase and superoxide dismutase, especially the CuZn superoxide dismutase isoform) and high, but transient elevation of catalase was found in inoculated leaves of resistant tobacco plants but not in susceptible plants.