Dynamics of tissue neutrophil sequestration after cutaneous burns in rats
Dynamics of tissue neutrophil sequestration after cutaneous burns in rats
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DOI：
10.1006/jsre.2000.5955
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发表时间：
2000-09-01
影响因子：
2.2
通讯作者：
Berthiaume, F
中科院分区：
文献类型：
--
作者：
Baskaran, H;Yarmush, ML;Berthiaume, F
背景碰撞后器官中的中性粒细胞募集可能导致局部血管损伤，这可以通过阻断中性粒细胞粘附到血管壁的药物来减少。由于这些药物可能增加感染的易感性，因此重要的是表征中性粒细胞隔离的动力学，以优化最终的抗粘连治疗。将大鼠烧伤超过其总体表面积（TBSA）的20%或40%，用盐水复苏。使用假手术对照。烧伤后1周，检测肺、肝、肾、肠和烧伤皮肤髓过氧化物酶（MPO）活性。烧伤后12 h，检测血管外I-125标记牛血清白蛋白（I-125-BSA）的含量。肺、肝、肾组织MPO活性在伤后3 h内升高，24-48 h恢复正常。峰值MPO水平发生在烧伤后6-12小时，两种烧伤面积相似。肠组织MPO无明显升高。烧伤皮肤MPO水平在6小时前没有增加，在24小时达到峰值，在48小时下降，但在7天内保持升高。组织化学证实肺和肝中的神经元募集。肾脏中未发现中性粒细胞。血管外I-125-BSA在肺、肝、肾和肠中增加，仅在40%TBSA组中增加。远端器官中的中性粒细胞隔离是一种短暂的现象，而中性粒细胞归巢到伤口部位是持续的。中性粒细胞积聚动力学与烧伤面积无关，尽管需要最小面积才能触发血管损伤，但可能采用临时早期抗粘连治疗来减少肺和肝中性粒细胞隔离，同时对中性粒细胞归巢至烧伤创面的影响很小。(C)北京大学出版社.
Background. Neutrophil recruitment in organs after bumps may cause local vascular damage, which can be reduced by agents blocking neutrophil adhesion to the vascular wall. Because these agents may increase susceptibility to infection, it is important to characterize the dynamics of neutrophil sequestration in order to optimize an eventual anti-adhesion therapy.Materials and methods. Rats were scald burned over 20 or 40% of their total body surface area (TBSA) and saline resuscitated. Sham controls were used. Myeloperoxidase (MPO) activity was measured in lungs, liver, kidney, gut, and burned skin up to 1 week postburn. Extravascular accumulation of I-125-labeled bovine serum albumin (I-125-BSA) was measured at 12 h postburn.Results. MPO activity in lungs, liver, and kidney was increased within 3 h postburn and returned to normal within 24-48 h. Peak MPO levels occurred at 6-12 h postburn and were similar for both burn sizes. No MPO increase was observed in gut. MPO levels in burned skin did not increase before 6 h, peaked at 24 h, decreased at 48 h, but remained elevated for up to 7 days. Neutrophil recruitment in lungs and liver was cofirmed histochemically. No neutrophils were found in kidneys. Extravascular I-125-BSA was increased in lungs, liver, kidneys, and gut, in the 40% TBSA group only.Conclusions. Neutrophil sequestration in remote organs is a transient phenomenon while neutrophil homing into the wound site is sustained. Neutrophil accumulation dynamics are independent of burn size, although a minimum size is required to trigger vascular damage, Temporary early anti-adhesion therapy to reduce lung and liver neutrophil sequestration with little impact on neutrophil homing into the burn wound may be possible. (C) 2000 Academic Press.