Expression of steroid receptor coactivator-1 is elevated during neuronal differentiation of murine neural stem cells
Expression of steroid receptor coactivator-1 is elevated during neuronal differentiation of murine neural stem cells
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DOI：
10.1016/j.brainres.2006.12.026
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发表时间：
2007-03
期刊：
影响因子：
2.9
通讯作者：
E. Nishihara;Takahiro Moriya;K. Shinohara
中科院分区：
文献类型：
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作者：
E. Nishihara;Takahiro Moriya;K. Shinohara
神经干细胞（NSCs）的细胞谱系特异性命运是由各种转录因子及其共调控因子（包括共激活因子和共抑制因子）调控的时空基因表达所决定的。为了阐明类固醇受体共激活因子-1（一种配体依赖性核受体共激活因子）在神经发生过程中的细胞分布，我们检测了SRC-1在小鼠胚胎神经节隆起来源的NSCs增殖和分化过程中的表达谱。我们发现SRC-1在增殖细胞和具有NSCs典型特征的多能前体细胞中很少表达。在促进NSCs分化的条件下，SRC-1转录物和蛋白水平均升高，成熟神经元中SRC-1阳性细胞的数量高于未成熟神经元。相比之下，SRC-1的表达很少局限于胶质系细胞，包括星形胶质细胞和少突胶质细胞。这些结果表明，SRC-1的表达伴随着多能性NSCs衍生的神经元命运承诺细胞的出现，并在NSCs分化过程中优先表达于神经元谱系细胞。
The cell-lineage-specific fate of neural stem cells (NSCs) is defined by spatial and temporal gene expression regulated by various transcription factors and their coregulators, including coactivators and corepressors. To clarify the cellular distribution of the steroid receptor coactivator-1 (SRC-1), a ligand-dependent nuclear receptor coactivator, during neurogenesis, we examined the expression profiles of SRC-1 during the proliferation and differentiation in culture of NSCs derived from the ganglionic eminence of mouse embryos. We found that SRC-1 was rarely expressed in proliferating cells and multipotent precursors with the typical characters of NSCs. Under conditions that promote the differentiation of NSCs, both the SRC-1 transcript and protein levels were elevated and the population of SRC-1-positive cells was found to be higher in the mature neurons than in the immature neurons. In contrast, SRC-1 expression was rarely localized in the glial lineage cells, including astrocytes and oligodendrocytes. These results indicate that SRC-1 expression accompanies the appearance of neuronal-fate-committed cells derived from multipotent NSCs and is preferentially expressed in neuronal lineage cells during the differentiation of NSCs.