Karyotype instability in Cryptococcus neoformans infection
Karyotype instability in Cryptococcus neoformans infection
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DOI：
10.1128/jcm.34.6.1531-1534.1996
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发表时间：
1996-06-01
影响因子：
9.4
通讯作者：
Casadevall, A
中科院分区：
文献类型：
--
作者：
Fries, BC;Chen, FY;Casadevall, A
应用等高线钳制均相电泳法对32株临床分离的新生隐球菌和3株环境分离的新生隐球菌进行了核型分析。不同患者分离株的核型有较大差异。序列分离自慢性或复发性感染患者的新生葡萄球菌具有非常相似的核型。然而，来自50%的患者的连续分离株之间检测到了微小的核型差异，表明体内发生了染色体重排或缺失。通过感染小鼠，从小鼠器官中回收分离株，并比较传代前后的核型，对三个临床株和三个环境株在小鼠体内传代前后的核型进行了研究。在小鼠传代后，检测到一个临床株和两个环境株的核型差异，我们的结果表明：(1)来自小地理区域的分离株之间存在广泛的核型差异；(2)个别患者的连续分离株之间存在较高频率的电泳核型差异；(3)在实验感染小鼠的过程中，发生了电泳核型的变化。
The electrophoretic karyotypes of 32 clinical and 3 environmental Cryptococcus neoformans isolates from New York City were studied by contour clamped homogeneous electrophoresis. There was extensive variation among the electrophoretic karyotypes of isolates from different patients. Sequential C. neoformans isolates from patients with chronic or relapsing infection had very similar karyotypes. However, minor differences in electrophoretic karyotypes were detected among sequential isolates from 50% of the patients studied, suggesting the occurrence of chromosomal rearrangements or deletions in vivo, This hypothesis was tested by infecting mice, recovering isolates from mouse organs, and comparing the electrophoretic karyotypes before and after passage, Three clinical and three environmental strains were studied before and after passage in mice. Karyotype differences were detected after mouse passage for one clinical and two environmental strains, Our results indicate (i) extensive karyotype variation among isolates from a small geographic region, (ii) a high frequency of electrophoretic karyotype differences among sequential isolates from individual patients, and (iii) the occurrence of electrophoretic karyotype changes during experimental infection of mice, The implications of these observations are discussed.