Preparation of organotypic hippocampal slice cultures: interface method
Preparation of organotypic hippocampal slice cultures: interface method
复制标题
DOI：
10.1038/nprot.2006.228
复制
发表时间：
2006-01-01
期刊：
影响因子：
14.8
通讯作者：
Yu, Lily My
中科院分区：
文献类型：
--
作者：
De Simoni, Anna;Yu, Lily My
该协议描述了一种在膜插入物上制作和培养大鼠海马器官切片的方法。包含补充视频以直观地演示该过程的不同步骤。培养的海马切片已越来越多地用作大脑突触研究的模型，因为它们允许在保留海马总体细胞结构的制剂中检查中长期操作。结合分子生物学、电生理学和免疫组织化学等技术来研究生理或病理过程可以很容易地应用于器官切片。这里描述的技术可用于从大脑的其他部分、其他啮齿动物物种和各个年龄段制作器官切片。该协议可在 3 小时内完成。
This protocol describes a method for making and culturing rat hippocampal organotypic slices on membrane inserts. Supplementary videos are included to demonstrate visually the different steps of the procedure. Cultured hippocampal slices has been increasingly used as a model for synaptic studies of the brain as they allow examination of mid to long term manipulations in a preparation where the gross cytoarchitecture of the hippocampus is preserved. Combining techniques such as molecular biology, electrophysiology and immunohistochemistry to study physiological or pathological processes can easily be applied to organotypic slices. The technique described here can be used to make organotypic slices from other parts of the brain, other rodent species and from a range of ages. This protocol can be completed in 3 h.