Condensin II initiates sister chromatid resolution during S phase.
Condensin II initiates sister chromatid resolution during S phase.
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DOI：
10.1083/jcb.201208008
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发表时间：
2013-02-18
期刊：
影响因子：
--
通讯作者：
Hirano T
中科院分区：
文献类型：
--
作者：
Ono T;Yamashita D;Hirano T
凝聚素II在S期启动复制染色体的结构重组，为有丝分裂过程中染色体的适当凝聚和分离做准备。凝聚素I和II是多亚单位复合体，在有丝分裂的染色体凝聚和分离中发挥着重要而独特的功能。与凝聚素I不同，凝聚素II在间期定位于细胞核，但在有丝分裂进入之前，凝聚素II可能具有什么功能仍知之甚少。在此，我们报道了凝聚素II在S期改变其染色质结合性质。值得注意的是，先进的染色体过早凝聚分析使我们能够在S期细胞的染色体复制区看到凝集素II形成“姊妹轴”。在S期，凝聚素II的耗尽影响了PCC驱动的姐妹染色单体的分辨率。此外，荧光原位杂交分析表明，在S期，凝聚素II而不是凝聚素I促进了重复染色体座位的分离。轻度复制应激的应用部分削弱了这一过程，并进一步加剧了凝聚素II耗尽所产生的表型。我们的结果表明，凝聚素II在S期启动了重复染色体的结构重组，为有丝分裂过程中染色体的适当凝聚和分离做准备。
Condensin II initiates structural reorganization of duplicated chromosomes during S phase to prepare for their proper condensation and segregation in mitosis. Condensins I and II are multisubunit complexes that play essential yet distinct functions in chromosome condensation and segregation in mitosis. Unlike condensin I, condensin II localizes to the nucleus during interphase, but it remains poorly understood what functions condensin II might have before mitotic entry. Here, we report that condensin II changes its chromatin-binding property during S phase. Remarkably, advanced premature chromosome condensation (PCC) assays enabled us to visualize condensin II forming “sister axes” in replicated regions of chromosomes in S phase cells. Depletion of condensin II compromised PCC-driven sister chromatid resolution during S phase. Moreover, fluorescence in situ hybridization assays revealed that condensin II, but not condensin I, promotes disjoining duplicated chromosomal loci during S phase. Application of mild replicative stress partially impaired this process and further exacerbated phenotypes arising from condensin II depletion. Our results suggest that condensin II initiates structural reorganization of duplicated chromosomes during S phase to prepare for their proper condensation and segregation in mitosis.
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DOI：
10.1073/pnas.85.4.1086
发表时间：
1988-02-01
影响因子：
11.1
作者：
HECK, MMS;HITTELMAN, WN;EARNSHAW, WC
通讯作者：
EARNSHAW, WC
影响因子：
7.5
作者：
Belmont, Andrew S.
通讯作者：
Belmont, Andrew S.
影响因子：
64.8
作者：
JOHNSON, RT;RAO, PN
通讯作者：
RAO, PN
影响因子：
7.8
作者：
Kireeva, N;Lakonishok, M;Belmont, AS
通讯作者：
Belmont, AS
影响因子：
64.8
作者：
KITSBERG, D;SELIG, S;CEDAR, H
通讯作者：
CEDAR, H