EXPRESSION OF MEMBRANE-ANTIGENS ON HUMAN ALVEOLAR MACROPHAGES AFTER EXPOSURE TO NITROGEN-DIOXIDE
EXPRESSION OF MEMBRANE-ANTIGENS ON HUMAN ALVEOLAR MACROPHAGES AFTER EXPOSURE TO NITROGEN-DIOXIDE
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DOI：
10.3109/08958379409003044
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发表时间：
1994-11-01
影响因子：
2.1
通讯作者：
UTELL, MJ
中科院分区：
文献类型：
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作者：
GAVRAS, JB;FRAMPTON, MW;UTELL, MJ
来自动物和人类调查的证据表明，暴露在二氧化氮(NO2)这种常见的空气污染物中会增加呼吸道感染的易感性。在动物中，暴露于NO2后，肺泡巨噬细胞(AM)的抗菌功能受到损害。我们试图确定人类暴露在NO2中是否导致不成熟的AM流入牙槽间隙和/或导致AM表面标记的表达减少，这些标记在抗菌防御中至关重要。8名志愿者在暴露于2.0ppm NO2或过滤空气6小时后立即接受了支气管肺泡灌洗(BAL)。免疫荧光染色和流式细胞术检测免疫球蛋白Fc组分(Ig)、补体受体3(CD11b)和单核细胞标志CD14受体在AM中的表达。内部荧光标准的使用将分析可变性降低了32%，并允许检测到细胞表面抗原表达的相对较小的变化。我们观察到CD11b的AM表达从空气中相当于可溶性荧光色素(MESF)的1.44(SE 0.19)×10(5)分子增加到NO2暴露后的1.78(SE 0.21)×10(5)Mese(p=0.04)。经非特异性抗体结合校正后，差异不再具有统计学意义(p=.059)。在细胞类型、大小或分化标志物的表达方面没有观察到明显的变化。2.0ppm NO2暴露6h不会降低AM中3种Fc受体CD11b或CD14的表达，也不会导致单核细胞在暴露后立即流入肺泡备用。
Evidence from both animal and human investigations suggests that exposure to nitrogen dioxide (NO2), a common air pollutant, increases susceptibility to respiratory infections. In animals, alveolar macrophage (AM) antimicrobial functions are impaired following NO2 exposure. We sought to determine whether exposure to NO2 in humans causes an influx of less mature AM into the alveolar space and/or results in decreased expression of AM surface markers important in antimicrobial defense. Eight human volunteers underwent bronchoalveolar lavage (BAL) immediately following 6-h exposures to 2.0 ppm NO2 or filtered air. AM expression of receptors for the Fc component of immunoglobulin (Ig), complement receptor 3 (CD11b), and monocyte marker CD14 were assessed using immunofluorescence staining and flow cytometry. The use of internal fluorescence standards reduced analytical variability by 32% and allowed detection of relatively small changes in cell surface antigen expression. We observed an increase in AM expression of CD11b from 1.44 (SE 0.19) x 10(5) molecules oi equivalent soluble fluorochrome (MESF) after air to 1.78 (SE 0.21) x 10(5) MESE after NO2 exposure (p=.04). When corrected for nonspecific antibody binding, the difference was no longer statistically significant (p=.059). No significant changes were observed in cell type, size, or expression of markers of differentiation. Exposure to 2.0 ppm NO2 for 6 h does not reduce AM expression of the 3 Fc receptors, CD11b, or CD14, and does not cause an influx of monocytes into the alveolar spare immediately after exposure.