Clinical Evaluation of Corneal Epithelial Barrier Function with the Slit‐Lamp Fluorophotometer
Clinical Evaluation of Corneal Epithelial Barrier Function with the Slit‐Lamp Fluorophotometer
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DOI：
10.1097/00003226-199509000-00007
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发表时间：
1995-09
期刊：
影响因子：
2.8
通讯作者：
N. Yokoi;S. Kinoshita
中科院分区：
文献类型：
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作者：
N. Yokoi;S. Kinoshita
我们设计了一个简单的和定量的方法，用于临床评价角膜上皮屏障功能，使用裂隙灯荧光光度计，并将其应用于健康志愿者和干眼症患者。所用的荧光光度法如下：以非接触方式将3 μl 0.5%荧光素溶液应用于角膜。滴注后10分钟，冲洗眼表。20分钟后，测量角膜荧光强度并转化为荧光素浓度。角膜状况分为4个等级：角膜上任何地方均无浅表点状角膜病变（SPK）（0级）;中央角膜无SPK（1级），中央角膜轻度SPK（2级）和中央角膜重度SPK（3级）。在志愿者中，角膜状况分类为0级，荧光素摄取（ng/ml）为22.4 ± 16.9（平均值± SD，n = 86）。在干眼症中，荧光素摄取显示出根据SPK严重程度的显著增加：1级：96.4 ± 51.2（n = 13）; 2级：318.6 ± 146.0（n = 11）;和3级：1479.1 ± 671.9（n = 10）（每个p < 0.0005）。该方法操作简便，可用于干眼或其他眼表疾病的SPK分级。
We designed a simple and quantitative method for the clinical evaluation of corneal epithelial barrier function using a slit-lamp fluorophotometer and applied it to healthy volunteers and dry-eye patients. The fluorophotometric method used was as follows: Three μl of 0.5% fluorescein solution was applied to the cornea in the untouched fashion. Ten minutes after instillation, the ocular surface was washed. Twenty minutes afterward, corneal fluorescence intensity was measured and converted into fluorescein concentration. Corneal condition was classified into four grades: no superficial punctate keratopathy (SPK) anywhere on the cornea (grade 0); no SPK at the central cornea (grade 1), mild SPK at the central cornea (grade 2), and severe SPK at the central cornea (grade 3). In the volunteers, corneal condition was classified as grade 0 and fluorescein uptake (ng/ml) was 22.4 ± 16.9 (mean ± SD, n = 86). In dry eye, fluorescein uptakes showed significant increase in accordance with SPK severity: grade 1: 96.4 ± 51.2 (n = 13); grade 2: 318.6 ± 146.0 (n = 11); and grade 3: 1479.1 ± 671.9 (n = 10) (each p < 0.0005). This simple method with the slit-lamp fluorophotometer can be used for the clinical grading of SPK in dry eye or other ocular surface diseases.