An in vivo dual-reporter system of cyanobacteria using two railroad-worm luciferases with different color emissions
An in vivo dual-reporter system of cyanobacteria using two railroad-worm luciferases with different color emissions
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DOI：
10.1093/pcp/pch001
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发表时间：
2004-01-01
影响因子：
4.9
通讯作者：
Oyama, T
中科院分区：
文献类型：
--
作者：
Kitayama, Y;Kondo, T;Oyama, T
使用荧光素酶的活体遗传报告系统能够实时监测活细胞中的基因表达。我们挑战了在同一细胞内同时监测两个独立启动子的活动，以准确地比较它们在体内的特征。在这份报告中，我们描述了一个简单的双报告系统，能够同时监测活的蓝藻细胞中两个启动子的活性。以催化不同颜色生物发光的两种铁路蠕虫荧光素酶为双报告分子，利用干涉滤光片成功分离出每种荧光，并利用光电倍增管估计单个生物发光信号。利用这个系统，我们清楚地展示了同一细胞中不同启动子之间的表达差异。
In vivo genetic reporter systems using luciferase enzymes enable the real-time monitoring of gene expression in living cells. We have challenged concurrent monitoring of two independent promoter activities within the same cells to precisely compare their characteristics in vivo. In this report, we describe a simple dual-reporter system capable of simultaneously monitoring two promoter activities in living cyanobacterial cells. Two railroad-worm luciferases catalyzing the bioluminescent emissions of different colors served as the dual reporters; each emission was successfully separated by interference filters to estimate the individual bioluminescence signals using photomultiplier tubes. Using this system, we clearly demonstrated the difference in the expression profiles between promoters in the same cells.