Response to diet-induced obesity produces time-dependent induction and progression of metabolic osteoarthritis in rat knees
Response to diet-induced obesity produces time-dependent induction and progression of metabolic osteoarthritis in rat knees
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DOI：
10.1002/jor.23103
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发表时间：
2016-06-01
影响因子：
2.8
通讯作者：
Herzog, Walter
中科院分区：
文献类型：
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作者：
Collins, Kelsey H.;Hart, David A.;Herzog, Walter
肥胖和相应的慢性低度炎症与膝关节炎的发病和进展有关。这种炎症的起源尚不清楚。本研究在12周的肥胖诱导和16周的适应期后，评估高脂高糖饮食诱导的肥胖（DIO）对局部（滑液）和全身（血清）炎症的影响。在12周的肥胖诱导中，n=40只DIO雄性Sprague-Dawley大鼠食用HFS饮食，而对照组（n=14）继续食用食物。12周时，将DIO大鼠分为俯卧组（DIO- p，根据体重变化前33%）和抵抗组（DIO- r，下33%）。动物在12岁和另外16周（28周）的饮食后被安乐死。在献祭时，收集和评估身体成分和膝关节。使用细胞因子阵列分析对滑液和血清进行分析。12周时，DIO-P动物的Modified Mankin评分高于DIO-R和chow (p=0.026)， DIO-R动物的Modified Mankin评分高于chow （p=0.049）。虽然DIO动物在12周时与chow相比，许多全身和有限的滑膜液炎症标志物增加，但到28周时，全身差异有限，但局部滑膜液炎症标志物浓度明显增加。代谢性OA可能表现为最初的全身性炎症紊乱。12周的肥胖诱导会导致独特的炎症特征和代谢性OA的诱导，在接下来的16周的肥胖和HFS饮食摄入后会发生改变，这表明肥胖是一个动态的、渐进的过程。(c) 2015年骨科研究会。Wiley期刊公司出版。[J]中华骨科杂志，2016,34(4):1010-1018。
Obesity, and corresponding chronic-low grade inflammation, is associated with the onset and progression of knee OA. The origin of this inflammation is poorly understood. Here, the effect of high fat, high sucrose (HFS) diet induced obesity (DIO) on local (synovial fluid), and systemic (serum) inflammation is evaluated after a 12-week obesity induction and a further 16-week adaptation period. For 12-weeks of obesity induction, n=40 DIO male Sprague-Dawley rats consumed a HFS diet while the control group (n=14) remained on chow. DIO rats were allocated to prone (DIO-P, top 33% based on weight change) or resistant (DIO-R, bottom 33%) groups at 12-weeks. Animals were euthanized at 12- and after an additional 16-weeks on diet (28-weeks). At sacrifice, body composition and knee joints were collected and assessed. Synovial fluid and sera were profiled using cytokine array analysis. At 12-weeks, DIO-P animals demonstrated increased Modified Mankin scores compared to DIO-R and chow (p=0.026), and DIO-R had higher Mankin scores compared to chow (p=0.049). While numerous systemic and limited synovial fluid inflammatory markers were increased at 12-weeks in DIO animals compared to chow, by 28-weeks there were limited systemic differences but marked increases in local synovial fluid inflammatory marker concentrations. Metabolic OA may manifest from an initial systemic inflammatory disturbance. Twelve weeks of obesity induction leads to a unique inflammatory profile and induction of metabolic OA which is altered after a further 16-weeks of obesity and HFS diet intake, suggesting that obesity is a dynamic, progressive process. (c) 2015 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 34:1010-1018, 2016.