The loss of the CBX7 gene expression represents an adverse prognostic marker for survival of colon carcinoma patients
The loss of the CBX7 gene expression represents an adverse prognostic marker for survival of colon carcinoma patients
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DOI：
10.1016/j.ejca.2010.05.011
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发表时间：
2010-08-01
影响因子：
8.4
通讯作者：
Tornillo, Luigi
中科院分区：
文献类型：
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作者：
Pallante, Pierlorenzo;Terracciano, Luigi;Tornillo, Luigi
我们先前已经表明，CBX 7表达与甲状腺癌中更恶性的表型相关。在此基础上，我们决定研究其在结直肠癌（CRC）中可能的预后价值。通过免疫组织化学分析了从大量散发性CRC切除（n = 1420）获得的组织微阵列（TMA）标本中的CBX 7表达。CBX 7表达数据与几个临床病理参数相关。与正常结肠粘膜相比，CBX 7表达在大量CRC样品中减少或不存在，并且CBX 7表达的丧失与CRC的不良结果相关（p < 0.001）。CBX 7表达的阻断似乎发生在转录水平上，因为定量RT-PCR分析显示，与正常对应组织相比，CRC样品中CBX 7特异性mRNA水平降低（高达50倍以上）。最后，两个CRC细胞系中CBX 7表达的恢复降低了它们的增殖率，这表明CBX 7表达的丧失在结肠癌发生的进展步骤中的作用。因此，这里报道的数据表明，CBX 7表达的评估可能代表结肠癌预后的有效工具，因为CRC患者的存活率降低与CBX 7表达的丧失相关。(C)2010爱思唯尔有限公司版权所有。
We have previously shown that CBX7 expression is associated with a more malignant phenotype in thyroid cancer. On this basis, we decided to investigate its possible prognostic value in colorectal cancer (CRC). CBX7 expression has been analysed by immunohistochemistry in tissue microarray (TMA) specimens obtained from a large series of sporadic CRC resections (n = 1420). The CBX7 expression data have been correlated with several clinico-pathological parameters. CBX7 expression is reduced or absent in a significant number of CRC samples in comparison to the normal colonic mucosa and the loss of CBX7 expression correlates with a poor outcome of CRC (p < 0.001). The block of CBX7 expression seems to occur at a transcriptional level since quantitative RT-PCR analysis showed a reduced CBX7-specific mRNA levels in CRC samples versus normal counterpart tissue (up to more than 50-fold). Finally, the restoration of CBX7 expression in two CRC cell lines reduces their proliferation rate suggesting a role of the loss of CBX7 expression in the progression step of colon carcinogenesis.Therefore, the data reported here indicate that the evaluation of CBX7 expression may represent a valid tool in the prognosis of colon cancer since a reduced survival of CRC patients is associated with the loss of CBX7 expression. (C) 2010 Elsevier Ltd. All rights reserved.