In vitro effects of S12911-2 on osteoclast function and bone marrow macrophage differentiation
In vitro effects of S12911-2 on osteoclast function and bone marrow macrophage differentiation
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DOI：
10.1016/s0014-2999(02)02040-x
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发表时间：
2002-08-16
影响因子：
5
通讯作者：
Tsouderos, Y
中科院分区：
文献类型：
--
作者：
Baron, R;Tsouderos, Y
为了确定 5-[双(羧甲基)氨基]-2-羧基4-氰基-3-噻吩乙酸二锶盐 (S12911-2) 是否通过作用于破骨细胞的分化和/或功能来抑制骨吸收，评估了其对 1,25-二羟基维生素 D-3 诱导的鸡骨髓细胞中碳酸酐酶 II 和玻连蛋白受体表达的影响，以及对骨吸收的影响。在骨切片上培养的真实大鼠破骨细胞的吸收活性。暴露 6 天后，S12911-2 剂量依赖性地抑制受刺激破骨细胞中碳酸酐酶 II 和玻连蛋白受体的表达（在 10(-3) M Sr2+、P 条件下分别为 46% 和 40%）
In order to determine whether 5-[bis(carboxymethyl) amino]-2-carboxy4-cyano-3-thiopheneacetic acid distrontium salt (S12911-2) inhibits bone resorption by acting on the differentiation and/or function of osteoclasts, its effects were assessed on the 1,25-dihydroxyvitamin D-3-induced expression of carbonic anhydrase II and vitronectin receptor in chicken bone marrow cells, and on the resorbing activity of authentic rat osteoclasts cultured on bone slices. S12911-2 dose-dependently inhibited, after a 6-day exposure, the expression of carbonic anhydrase II and vitronectin receptor in stimulated osteoclasts (46% and 40%, respectively, at 10(-3) M Sr2+, P