Combining in vitro and in vivo screening to identify efficient Pseudomonas biocontrol strains against the phytopathogenic bacterium Ralstonia solanacearum.
Combining in vitro and in vivo screening to identify efficient Pseudomonas biocontrol strains against the phytopathogenic bacterium Ralstonia solanacearum.
复制标题
DOI：
10.1002/mbo3.1283
复制
发表时间：
2022-04
期刊：
影响因子：
3.4
通讯作者：
Friman, Ville-Petri
中科院分区：
文献类型：
--
作者：
Clough, Sophie E.;Jousset, Alexandre;Elphinstone, John G.;Friman, Ville-Petri
关键词：
虽然传统上使用合成农用化学品控制植物病原体，但商业杀菌剂的可用性仍然有限。一种潜在的控制策略可能是利用植物生长促进菌（PGPB）通过资源竞争或生产抗菌化合物来抑制病原体。本研究旨在对8株假单胞菌进行抗青枯病病原菌（Ralstonia solanacearum）的体外和体内筛选，并探讨其抑制病原菌的潜在机制。我们发现抑制作用是假单胞菌菌株特异性的，菌株CHA0表现出最高的病原体抑制作用。基因组筛选在最具抑制作用的菌株基因组中鉴定出2,4‐二乙酰间苯三酚、pyoluteorin和orfamides A和B次生代谢物簇，并对其进行进一步研究。虽然这些化合物都能抑制番茄红霉的生长，但质谱分析表明，生长培养基中只产生orfamide A。此外，从假单胞菌培养物中提取的orfamide变体显示出高度的病原体抑制作用。以“Micro - Tom”番茄品种为研究对象，发现CHA0能降低其中一种病原菌的青枯病发病率。总之，这些发现表明，需要更好地了解假单胞菌-拉尔斯顿菌在根际的相互作用，才能成功地将体外研究结果转化为农业应用。本研究旨在对8株假单胞菌进行抗青枯病病原菌（Ralstonia solanacearum）的体外和体内筛选，并探讨其抑制病原菌的潜在机制。基因组筛选鉴定出2,4‐二乙酰间苯三酚、pyoluteorin和orfamides A和B次生代谢物簇，它们在最具抑制作用的菌株基因组中都抑制了R. solanacearum的生长。虽然假单胞菌可以降低番茄细菌性枯萎病的发病率，但其效果取决于番茄青霉菌株，因此需要更好地了解假单胞菌与拉尔斯顿菌在根际的相互作用。
Although plant pathogens are traditionally controlled using synthetic agrochemicals, the availability of commercial bactericides is still limited. One potential control strategy could be the use of plant growth‐promoting bacteria (PGPB) to suppress pathogens via resource competition or the production of antimicrobial compounds. This study aimed to conduct in vitro and in vivo screening of eight Pseudomonas strains against Ralstonia solanacearum (the causative agent of bacterial wilt) and to investigate underlying mechanisms of potential pathogen suppression. We found that inhibitory effects were Pseudomonas strain‐specific, with strain CHA0 showing the highest pathogen suppression. Genomic screening identified 2,4‐diacetylphloroglucinol, pyoluteorin, and orfamides A and B secondary metabolite clusters in the genomes of the most inhibitory strains, which were investigated further. Although all these compounds suppressed R. solanacearum growth, only orfamide A was produced in the growth media based on mass spectrometry. Moreover, orfamide variants extracted from Pseudomonas cultures showed high pathogen suppression. Using the “Micro‐Tom” tomato cultivar, it was found that CHA0 could reduce bacterial wilt disease incidence with one of the two tested pathogen strains. Together, these findings suggest that a better understanding of Pseudomonas–Ralstonia interactions in the rhizosphere is required to successfully translate in vitro findings into agricultural applications. This study aimed to conduct in vitro and in vivo screening of eight Pseudomonas strains against Ralstonia solanacearum (the causative agent of bacterial wilt) and to investigate underlying mechanisms of potential pathogen suppression. Genomic screening identified 2,4‐diacetylphloroglucinol, pyoluteorin, and orfamides A and B secondary metabolite clusters in the genomes of the most inhibitory strains, which all suppressed R. solanacearum growth. Although Pseudomonas could reduce bacterial wilt disease incidence with tomato, the effect depended on the R. solanacearum strain, highlighting the need to better understand Pseudomonas–Ralstonia interactions in the rhizosphere.
登录
查看更多内容
影响因子：
8.8
作者：
Becker, Joachim;Eisenhauer, Nico;Jousset, Alexandre
通讯作者：
Jousset, Alexandre
影响因子：
5.2
作者：
Almario, Juliana;Bruto, Maxime;Muller, Daniel
通讯作者：
Muller, Daniel
影响因子：
4.1
作者：
Chaiharn, Mathurot;Chunhaleuchanon, Somporn;Lumyong, Saisamorn
通讯作者：
Lumyong, Saisamorn
影响因子：
3.2
作者：
Clarke, Christopher R.;Studholme, David J.;Vinatzer, Boris A.
通讯作者：
Vinatzer, Boris A.
影响因子：
5.2
作者：
Flury P;Vesga P;Péchy-Tarr M;Aellen N;Dennert F;Hofer N;Kupferschmied KP;Kupferschmied P;Metla Z;Ma Z;Siegfried S;de Weert S;Bloemberg G;Höfte M;Keel CJ;Maurhofer M
通讯作者：
Maurhofer M