Regulation of pancreatic duct epithelial growth in vitro.
Regulation of pancreatic duct epithelial growth in vitro.
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体外胰管上皮生长的调节。
DOI：
10.1152/ajpgi.1990.258.6.g833
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发表时间：
1990
期刊：
影响因子：
--
通讯作者：
Hootman,SR
中科院分区：
文献类型：
--
作者：
Verme,TB;Hootman,SR
研究了几种可能的营养因子对豚鼠胰管上皮单层细胞生长的影响。制备离体小叶导管和小叶间管片段，分别移植到组织培养塑料和I型胶原厚凝胶上。从外植体中生长出的单层首先在基础培养基中培养3或4天。然后，在培养基中分别添加糖素、碳醇、蛋白、表皮生长因子（EGF）、分泌素、12- o -十四烷酰磷13-乙酸酯（TPA）或血管活性肠肽（VIP）。在没有或存在这些可能的营养因子的情况下培养细胞，并在0、2和4天对单层面积进行形态测定。增长率由每2天的面积增加来确定。在4天的试验期内，单分子膜在塑料上的生长面积增加到初始面积的479%；那些生长在胶原蛋白上的增加到了523%。在两种培养基上分别培养bombesin、carbachol、caerulein、secretin、TPA和VIP的外植体生长速率与在基础培养基上培养的外植体生长速率无显著差异。相比之下，在10 nM EGF存在下，生长在塑料或胶原蛋白上的导管单层面积分别扩大到初始面积的722%和1070%。这种营养效应的EC50约为1 nM。这些结果表明，EGF在体外对豚鼠胰管细胞具有强大的营养作用，但也表明胰腺主管和小叶间管的细胞分裂不受细小蛋白和相关肽激素的调节，而这些激素已被报道对体内外分泌胰腺具有促进生长的作用。
Effects of a number of possible trophic factors on growth of guinea pig pancreatic duct epithelial monolayers were investigated. Isolated fragments of main and interlobular ducts were prepared and explanted onto both tissue culture plastic and thick gels of type I collagen. Monolayers growing out from explants were first cultured in a basal medium for 3 or 4 days. Next, the medium was supplemented individually with bombesin, carbachol, caerulein, epidermal growth factor (EGF), secretin, 12-O-tetradecanoylphorbol 13-acetate (TPA), or vasoactive intestinal peptide (VIP). Cells were cultured in the absence or presence of these possible trophic factors, and monolayer areas were determined morphometrically at 0, 2, and 4 days. Rate of growth was determined from increase in area over each 2-day period. Monolayers grown in basal medium alone on plastic increased to 479% of initial area over the 4-day test period; those grown on collagen increased to 523%. Explants cultured in presence of bombesin, carbachol, caerulein, secretin, TPA, and VIP on either substrate grew at rates not significantly different from those cultured in basal medium. By contrast, duct monolayers grown on plastic or collagen in presence of 10 nM EGF expanded in area to 722 and 1,070%, respectively, of their initial areas. The EC50 for this trophic effect was approximately 1 nM. These results show that EGF exerts a potent trophic effect on guinea pig pancreatic duct cells in vitro but also indicate that cell division in the pancreatic main and interlobular ducts is not regulated by caerulein and related peptide hormones that have been reported to have growth-promoting effects on exocrine pancreas in vivo.