Cell sorting technique based on thermoresponsive differential cell adhesiveness.
Cell sorting technique based on thermoresponsive differential cell adhesiveness.
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DOI：
10.1021/bm070314f
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发表时间：
2007-07
影响因子：
6.2
通讯作者：
T. Matsuda;Yuko Saito;K. Shoda
中科院分区：
文献类型：
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作者：
T. Matsuda;Yuko Saito;K. Shoda
从不同细胞类型的混合物中分选出特定的靶细胞是基于干细胞和组织工程的功能性工程组织开发的先决条件。本文提出了一种新的细胞分选方法，该方法使用温敏细胞粘附和非细胞粘附物质的混合物。前者是聚（N-异丙基丙烯酰胺）接枝明胶（PNIPAM-明胶），后者是PNIPAM。通过以适当的混合比例混合这些温敏物质的涂层产生的分级细胞粘附，清楚地区分了两种牛血管细胞类型（内皮细胞和平滑肌细胞）的粘附潜力。如使用共聚焦激光扫描荧光显微镜所测定的，在室温下分离并随后在37 ° C下重新铺板在涂覆有具有与先前采用的相同组成的混合涂层的皿上的顺序程序产生了非常纯的靶细胞。该方法导致靶细胞的收获，其特征在于操作简单而无细胞损伤。这些优点有望促进干细胞和组织工程。
Cell sorting of specific target cells from a mixture of different cell types is a prerequisite for development of functional engineered tissues based on stem-cell and tissue engineering. This paper presents a new method of cell sorting that uses a mixture of thermoresponsive cell-adhesive and non-cell-adhesive substances. The former substance is poly(N-isopropylacrylamide)-grafted gelatin (PNIPAM-gelatin) and the latter is PNIPAM. Graded cell adhesion, produced by mixed coating of these thermoresponsive substances at an appropriate mixing ratio, clearly differentiated the adhesive potentials of two bovine vascular cell types (endothelial cell and smooth muscle cell). The sequential procedures of detachment at room temperature and subsequent replating at 37 degrees C on dishes coated with a mixed coating with the same composition as that employed previously yielded remarkably pure target cells, as determined using confocal laser scanning fluorescence microscopy. This method, leading to harvesting of target cells, is characteristic of simple manipulation with no cell damage. Such advantages are expected to facilitate stem-cell and tissue engineering.