Identification of a recessive gene YrZ15-1370 conferring adult plant resistance to stripe rust in wheat-Triticum boeoticum introgression line
Identification of a recessive gene YrZ15-1370 conferring adult plant resistance to stripe rust in wheat-Triticum boeoticum introgression line
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DOI：
10.1007/s00122-021-03866-3
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发表时间：
2021-03
影响因子：
5.4
通讯作者：
M. Zhang;X. Liu;T. Peng;D. Wang;D. Liang;H. Li;M. Hao;S. Ning;Z. Yuan;Bo Jiang;
中科院分区：
文献类型：
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作者：
M. Zhang;X. Liu;T. Peng;D. Wang;D. Liang;H. Li;M. Hao;S. Ning;Z. Yuan;Bo Jiang;
小麦条锈病是由条锈菌（Pucciniastriiformisf.）小麦赤霉病（Triticum boeoticum Boiss（T.单球菌属山羊草亚种，2n= 2x= 14，AbAb）登记G52赋予对中国流行类群混合物的高水平成株抗性。利用G52与感病普通小麦基因型Crocus杂交，将其抗性转移到普通小麦上。高抗小麦T.通过细胞学和Pstraces鉴定，选育出具有42条染色体的大豆渐渗系Z15-1370（F5代）。用Z15-1370与感条锈病的普通小麦明鲜169配制F_1、F_2、F_2：3代。遗传分析表明，Z15-1370的抗病性受一对隐性基因控制，暂定名为YrZ 15 -1370。利用BSR-Seq分析，将YrZ 15 - 1370定位于第6AL染色体上，并在4.3cM的遗传区间内（相当于1.8Mb的物理区域），两侧分别有KASP 1370 - 3和KASP-1370- 5标记。其中YrZ 15 - 15是一个抗病相关基因，1370与先前基于其位置和/或起源在染色体6A上鉴定的Yr基因不同。YrZ 15 - 1370是小麦抗病育种的重要资源，其侧翼标记可用于分子标记辅助选择（MAS）育种和进一步克隆该基因。
Key messageA novel recessive geneYrZ15-1370derived fromTriticum boeoticumconfers adult–plant resistance to wheat stripe rust.AbstractStripe rust, caused byPuccinia striiformisf. sp.tritici(Pst), is one of the most damaging diseases of wheat globally and resistance is the effectively control strategy.Triticum boeoticumBoiss (T. monococcumL. ssp.aegilopoides, 2n= 2x= 14, AbAb) accession G52 confers a high level of adult–plant resistance against a mixture of the Chinese prevalentPstraces. To transfer the resistance to common wheat, a cross was made between G52 and susceptible common wheat genotype Crocus. A highly resistant wheat-T. boeoticumintrogression line Z15-1370 (F5generation) with 42 chromosomes was selected cytologically and by testing withPstraces. F1, F2, and F2:3generations of the cross between Z15-1370 and stripe rust susceptible common wheat Mingxian169 were developed. Genetic analysis revealed that the resistance in Z15-1370 was controlled by a single recessive gene, tentatively designatedYrZ15-1370. Using the bulked segregant RNA-Seq (BSR-Seq) analysis,YrZ15-1370was mapped to chromosome 6AL and flanked by markersKASP1370-3andKASP-1370-5within a 4.3 cM genetic interval corresponding to 1.8 Mb physical region in the Chinese Spring genome, in which a number of disease resistance-related genes were annotated.YrZ15-1370differed from previouslyYrgenes identified on chromosome 6A based on its position and/or origin. TheYrZ15-1370would be a valuable resource for wheat resistance improvement and the flanking markers developed here could be useful tools for marker-assisted selection (MAS) in breeding and further cloning the gene.