Mechanical property characterization of electrospun recombinant human tropoelastin for vascular graft biomaterials.
Mechanical property characterization of electrospun recombinant human tropoelastin for vascular graft biomaterials.
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DOI：
10.1016/j.actbio.2011.08.001
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发表时间：
2012-01
影响因子：
9.7
通讯作者：
Gregory KW
中科院分区：
文献类型：
--
作者：
McKenna KA;Hinds MT;Sarao RC;Wu PC;Maslen CL;Glanville RW;Babcock D;Gregory KW
血管移植物的开发重点是寻找一种非血栓形成、最大限度地减少内膜增生、与自体血管的机械性能相匹配并允许动脉组织再生的生物材料。在这项研究中，电纺重组人弹性蛋白原（rTE）的结构和机械性能和血管细胞的相容性进行了评估，作为一个潜在的血管移植物的支持基质。使用辛二酸二琥珀酰亚胺酯（DSS）交联电纺rTE纤维以产生在水性环境中稳定的聚合物重组弹性蛋白原（prTE）基质。构造管状的1cm直径的prTE样品用于单轴拉伸测试，并且从15wt%rTE溶液制备4mm小直径的prTE管状支架用于爆裂压力和细胞相容性评价。单轴拉伸试验表明，平均极限拉伸强度（UTS）为0.36±0.05 MPa，弹性模量为0.15±0.04 MPa和0.91±0.16 MPa，与提取的天然弹性蛋白相当。从平均壁厚为453 ± 74 μm的4 mm ID支架获得485 ± 25 mmHg的爆破压力。prTE支持内皮细胞生长，在培养48小时后具有典型的内皮细胞鹅卵石形态。交联电纺重组人原弹性蛋白具有用作具有可定制尺寸、顺应性基质和血管细胞相容性的血管移植物生物材料的有前景的性质。
The development of vascular grafts has focused on finding a biomaterial that is non-thrombogenic, minimizes intimal hyperplasia, matches the mechanical properties of native vessels and allows for regeneration of arterial tissue. In this study, the structural and mechanical properties and the vascular cell compatibility of electrospun recombinant human tropoelastin (rTE) were evaluated as a potential vascular graft support matrix. Disuccinimidyl suberate (DSS) was used to cross-link electrospun rTE fibers to produce a polymeric recombinant tropoelastin (prTE) matrix that is stable in aqueous environments. Tubular 1 cm diameter prTE samples were constructed for uniaxial tensile testing and 4 mm small-diameter prTE tubular scaffolds were produced for burst pressure and cell compatibility evaluations from 15 wt% rTE solutions. Uniaxial tensile tests demonstrated an average ultimate tensile strength (UTS) of 0.36±0.05 MPa and elastic moduli of 0.15±0.04 MPa and 0.91±0.16 MPa, which were comparable to extracted native elastin. Burst pressures of 485 ± 25 mmHg were obtained from 4 mm ID scaffolds with 453 ± 74 μm average wall thickness. prTE supported endothelial cell growth with typical endothelial cell cobblestone morphology after 48 hours in culture. Cross-linked electrospun recombinant human tropoelastin has promising properties for utilization as a vascular graft biomaterial with customizable dimensions, a compliant matrix, and vascular cell compatibility.