Dietary selenium requirements of juvenile grouper, Epinephelus malabaricus
Dietary selenium requirements of juvenile grouper, Epinephelus malabaricus
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DOI：
10.1016/j.aquaculture.2005.03.022
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发表时间：
2005-11-14
期刊：
影响因子：
4.5
通讯作者：
Shiau, SY
中科院分区：
文献类型：
--
作者：
Lin, YH;Shiau, SY
研究了石斑鱼8周的饲粮硒需取量。试验1在基础饲粮中分别添加0、0.5、1、2、3和5 mg Se/kg硒，试验2在基础饲粮中添加0、0.1、0.2、0.4、0.6、1和2 mg Se/kg硒，分别提供0.21、0.77、1.38、2.02、2.70和4.00 mg Se/kg（试验1）和0.17、0.25、0.39、0.55、0.79、1.23和2.10 mg Se/kg（试验2）。试验1和试验2分别饲喂3个重复组（初始平均体重分别为12.20 +/- 0.14 g和11.94 +/- 0.15 g）。在饲养期间对饲养水中硒浓度进行监测，两项试验均未检测到硒浓度。试验1中，饲粮硒含量为0.77 mg /kg时增重和饲料效率最高（P < 0.05），其次为饲粮硒含量为2.02、2.70 mg /kg和未添加基础饲粮，饲粮硒含量为4.00 mg /kg时最低。肝脏谷胱甘肽过氧化物酶（GPx）活性以>= 2.02 mg Se/kg时最高，其次为0.77和1.38 mg Se/kg，基础饲料最低。基础饲粮的肝脏谷胱甘肽还原酶（GR）活性高于其他各组。在试验2中，硒含量为0.79和1.23 mg /kg的饲料比硒含量为0.25、0.55 mg /kg的饲料和基础饲料的增重和FE更高。肝脏GPx活性最高的饲料中添加：！硒含量为1.23 mg /kg，其次为0.79 mg Se/kg，其次为0.39和0.55 mg Se/kg，以基础饲料最低。基础饲粮中肝脏GR活性最高，其次为0.55 mg Se/kg, >= 0.39 mg Se/kg时肝脏GR活性最低。通过体重增加和鱼体硒保留量的线性回归估计，生长中的石斑鱼适宜的饲粮硒浓度约为0.7 mg硒/kg。(C) 2005 Elsevier B.V.版权所有
Two 8-week feeding experiments were conducted to estimate the dietary selenium (Se) requirement of grouper. Selenomethionine was added to the basal diet at 0, 0.5, 1, 2, 3 and 5 mg Se/kg in Exp. 1 and 0, 0.1, 0.2, 0.4, 0.6, 1 and 2 mg Se/kg in Exp. 2 providing 0.21, 0.77, 1.38, 2.02, 2.70 and 4.00 mg Se/kg (Exp. 1) and 0.17, 0.25, 0.39, 0.55, 0.79, 1.23 and 2.10 mg Se/kg (Exp. 2), respectively. Each diet was fed to triplicate groups of fish (mean initial weight: 12.20 +/- 0.14 g in Exp. 1 and 11.94 +/- 0.15 g in Exp. 2) in a closed, recirculating rearing system. The Se concentration in rearing water was monitored during the feeding period, and was not detectable in both experiments. In Exp. 1, weight gain and feed efficiency (FE) were highest (P < 0.05) in fish fed diet with 0.77 mg Se/kg, followed by fish fed diets with 2.02 and 2.70 mg Se/kg and the unsupplemented basal diet, and lowest in fish fed diet with 4.00 mg Se/kg. Hepatic glutathione peroxidase (GPx) activity was highest in fish fed diets with >= 2.02 mg Se/kg, followed by 0.77 and 1.38 mg Se/kg, and lowest in fish fed the basal diet. Fish fed the basal diet had higher hepatic glutathione reductase (GR) activity than fish in all the other dietary groups. In Exp. 2, weight gain and FE were higher in fish fed diets with 0.79 and 1.23 mg Se/kg than fish fed diets with 0.25, 0.55 mg Se/kg and the basal diet. Hepatic GPx activity was highest in fish fed diets with :! 1.23 mg Se/kg, followed by 0.79 mg Se/kg, then 0.39 and 0.55 mg Se/ kg, and lowest in fish fed the basal diet. Hepatic GR activity was highest in fish fed the basal diet, followed by 0.55 mg Se/kg, and lowest in fish fed diets with >= 0.39 mg Se/kg. The adequate dietary Se concentration for growing grouper estimated by weight gain and by linear regression of whole-body Se retention of the fish is about 0.7 mg Se/kg. (C) 2005 Elsevier B.V. All rights reserved.