Regulation of expression from the glnA promoter of Escherichia coli in the absence of glutamine synthetase.
Regulation of expression from the glnA promoter of Escherichia coli in the absence of glutamine synthetase.
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在没有谷氨酰胺合成酶的情况下，大肠杆菌 glnA 启动子的表达调节。
DOI：
10.1073/pnas.77.12.7372
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发表时间：
1980
影响因子：
11.1
通讯作者：
Magasanik,B
中科院分区：
文献类型：
--
作者：
Rothstein,DM;Pahel,G;Tyler,B;Magasanik,B
肠细菌中谷氨酰胺合成酶[L-谷氨酸：氨连接酶（ADP-形成），EC 6.3.1.2]的一种可疑调节剂是谷氨酰胺合成酶本身。我们分离出携带β-半乳糖苷酶结构基因与谷氨酰胺合成酶基因启动子融合的大肠杆菌菌株，借助Casadaban Mud 1（ApR，lac，cts 62）噬菌体。尽管缺乏谷氨酰胺合成酶，但在单倍体融合菌株中保留了某些方面的调节，而其他方面需要谷氨酰胺合成酶催化或调节活性或两者兼而有之。还确定了谷氨酰胺合成酶基因的转录方向。
One of the suspected regulators of glutamine synthetase [L-glutamate:ammonia ligase (ADP-forming), EC 6.3.1.2] in enteric bacteria is glutamine synthetase itself. We isolated Escherichia coli strains carrying fusions of the beta-galactosidase structural gene to the promoter of the glutamine synthetase gene, with the aid of the Casadaban Mud1 (ApR, lac, cts62) phage. Some aspects of regulation were retained in haploid fusion strains despite the absence of glutamine synthetase, whereas other aspects required glutamine synthetase catalytic or regulatory activity or both. The direction of transcription of the glutamine synthetase gene was also determined.