Molecular mechanisms of leukocyte recruitment in the inflammatory process.
Molecular mechanisms of leukocyte recruitment in the inflammatory process.
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DOI：
10.1016/s0008-6363(96)00066-1
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发表时间：
1996-10
影响因子：
10.8
通讯作者：
K. Ley
中科院分区：
文献类型：
--
作者：
K. Ley
微循环构成循环血液和组织间隙之间的功能界面。为了到达炎症部位，白细胞必须通过内皮屏障。募集模式包括白细胞边缘化、捕获、滚动、活化、牢固粘附和迁移。最近的实验研究表明，E-、L-、P-选择素和α 4整合素可以介导白细胞滚动。在趋化因子、补体肽或脂质介质的激活下，β 1和β 2整合素、细胞间粘附分子（ICAM）和血管细胞粘附分子-1（VCAM-1）提供牢固的粘附。β 1和β 2整合素以及血小板-内皮细胞粘附分子-1（PECAM-1）已被证明参与了细胞的迁移。活体显微镜技术在将细胞和分子生物学的概念性进展应用于体内情况方面发挥了重要作用。这篇综述着重于目前的理解，白细胞招募的范例，建议在体内观察和体外模型系统。中性粒细胞募集的范例被提出作为其他炎症细胞募集机制的模型。
The microcirculation constitutes the functional interface between the circulating blood and the interstitial space. To gain access to sites of inflammation, leukocytes must pass the endothelial barrier. The recruitment paradigm encompasses leukocyte margination, capture, rolling, activation, firm adhesion, and transmigration. Recent experimental work has shown that E-, L- and P-selectins and α4integrins can mediate leukocyte rolling. Upon activation by chemokines, complement peptides or lipid mediators, firm adhesion is afforded by β1and β2integrins, Intercellular Adhesion Molecules (ICAMs) and Vascular Cell Adhesion Molecule-1 (VCAM-1). β1and β2integrins as well as Platelet-Endothelial Cell Adhesion Molecule-1 (PECAM-1) have been shown to be involved in transmigration. Intravital microscopic techniques have been instrumental in applying the conceptual advances of cell and molecular biology to the in vivo situation. This review focuses on the current understanding of the leukocyte recruitment paradigm as suggested by in vivo observations and in vitro model systems. The paradigm of neutrophil recruitment is presented to serve as a model for the recruitment mechanisms of other inflammatory cells.