A COMMON TRANSCRIPTIONAL ACTIVATOR IS LOCATED IN THE CODING REGION OF 2 REPLICATION-DEPENDENT MOUSE HISTONE GENES
A COMMON TRANSCRIPTIONAL ACTIVATOR IS LOCATED IN THE CODING REGION OF 2 REPLICATION-DEPENDENT MOUSE HISTONE GENES
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DOI：
10.1128/mcb.11.6.2929
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发表时间：
1991-06-01
影响因子：
5.3
通讯作者：
MARZLUFF, WF
中科院分区：
文献类型：
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作者：
HURT, MM;BOWMAN, TL;MARZLUFF, WF
在小鼠组蛋白H3基因的蛋白编码序列中存在一个高表达所需的区域。 当将H3.2基因的密码子58至93的110个核苷酸的编码区激活序列（CRAS）以正确的方向放置在转录起始点5'的520个核苷酸时，恢复表达。 由于原始缺失基因和CRAS在-520的缺失基因的转录产生相同的mRNA分子，因此完全排除了缺失对mRNA稳定性或其他转录后事件的影响。 在H3基因的适当位置的CRAS序列的倒位只对表达产生三倍的影响。 在H2a.2基因的编码区中的框内缺失导致在该基因的高表达所必需的氨基酸50和85之间的编码区中鉴定出105个核苷酸的序列。 另外，将H3 CRAS插入H2a.2基因的缺失区域恢复了H2 a基因的表达。 因此，CRAS元件具有取向依赖性、位置无关性效应。 凝胶迁移率变化竞争研究表明，相同的蛋白质与H3和H2 a CRAS元件相互作用，这表明一个共同的因素参与组蛋白基因的表达。
There is a region in the mouse histone H3 gene protein-encoding sequence required for high expression. The 110-nucleotide coding region activating sequence (CRAS) from codons 58 to 93 of the H3.2 gene restored expression when placed 520 nucleotides 5' of the start of transcription in the correct orientation. Since identical mRNA molecules are produced by transcription of the original deletion gene and the deletion gene with the CRAS at -520, effects of the deletions on mRNA stability or other posttranscriptional events are completely ruled out. Inversion of the CRAS sequence in its proper position in the H3 gene resulted in only a threefold effect on expression. In-frame deletions in the coding region of an H2a.2 gene led to identification of a 105-nucleotide sequence in the coding region between amino acids 50 and 85 necessary for high expression of the gene. Additionally, insertion of the H3 CRAS into the deleted region of the H2a.2 gene restored expression of the H2a gene. Thus, the CRAS element has an orientation-dependent, position-independent effect. Gel mobility shift competition studies indicate that the same proteins interact with both the H3 and H2a CRAS elements, suggesting that a common factor is involved in expression of histone genes.