The dissociation rate of a winged helix protein-DNA complex is influenced by non-DNA contact residues.
The dissociation rate of a winged helix protein-DNA complex is influenced by non-DNA contact residues.
复制标题
有翼螺旋蛋白-DNA 复合物的解离速率受非 DNA 接触残基的影响。
DOI：
10.1006/abbi.1998.1040
复制
发表时间：
1999
期刊：
影响因子：
--
通讯作者：
Liao,X
中科院分区：
文献类型：
--
作者：
Shiyanova,T;Liao,X
The dissociation constants and the half-lives of the DNA complexes of winged helix protein Genesis and its mutants were investigated. Our data show that substitutions of non-DNA contact residues in the two highly dynamic wing sequences dramatically change the off rates of the complexes. Thus, our data indicate that the off rate of a DNA complex is encoded in the amino acid sequence of the DNA-binding protein and/or should be a unique property for a protein–DNA complex. Our data also indicate that the local structural transition of a DNA-binding protein in its DNA recognition process is likely to go through a transition state, which can control the on and off rates of a complex.
