Regulation of replication timing in fission yeast
Regulation of replication timing in fission yeast
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DOI：
10.1093/emboj/20.21.6115
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发表时间：
2001-11-01
期刊：
影响因子：
11.4
通讯作者：
Huberman, JA
中科院分区：
文献类型：
--
作者：
Kim, SM;Huberman, JA
在这里，我们报告了裂殖酵母裂殖酵母中复制时间及其调节的第一个特征。我们使用了三种不同的同步方法：离心淘洗、cdc10 温度变化和释放，以及通过用羟基脲 (HU) 处理然后去除 HU 来饥饿脱氧核糖核苷三磷酸 (dNTP)，以研究特定自主复制序列元件（ARS 元件；潜在复制起点）在 S 期复制的时间。我们发现各个 ARS 元素在特征时间复制，有的早，有的晚，与同步方法无关。在用 HU 处理的野生型细胞中，早期的 ARS 元件复制，但晚期的 ARS 元件没有复制。然而，在缺乏 Rad3（类似于人类 ATR 和 ATM）或 Cds1（类似于人类 CHK2）检查点激酶的 HU 处理的突变细胞中，早期和晚期 ARS 元件都能够复制。因此，在 dNTP 饥饿的条件下，需要 Rad3 和 Cds1 激酶来抑制正常晚期复制区域的复制。
Here we report the first characterization of replication timing and its regulation in the fission yeast Schizosaccharomyces pombe. We used three different synchronization methods: centrifugal elutriation, cdc10 temperature-shift and release, and starvation for deoxyribonucleoside triphosphates (dNTPs) by treatment with hydroxyurea (HU) followed by removal of HU, to study the times when specific autonomously replicating sequence elements (ARS elements; potential replication origins) replicate during S phase. We found that individual ARS elements replicate at characteristic times, some early and some late, independently of synchronization method. In wild-type cells treated with HU, early ARS elements replicated but late ones did not. However, in HU-treated mutant cells lacking the Rad3 (similar to human ATR and ATM) or Cds1 (similar to human CHK2) checkpoint kinase, both early and late ARS elements were able to replicate. Thus under conditions of dNTP starvation the Rad3 and Cds1 kinases are needed to suppress the replication of normally late-replicating regions.