Alkaline stress triggers an immediate calcium fluctuation in Candida albicans mediated by Rim101p and Crz1p transcription factors.
Alkaline stress triggers an immediate calcium fluctuation in Candida albicans mediated by Rim101p and Crz1p transcription factors.
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DOI：
10.1111/j.1567-1364.2011.00730.x
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发表时间：
2011-08
影响因子：
3.2
通讯作者：
Hui Wang;Yong Liang;Biao Zhang;Wen Zheng;L. Xing;Mingchun Li
中科院分区：
文献类型：
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作者：
Hui Wang;Yong Liang;Biao Zhang;Wen Zheng;L. Xing;Mingchun Li
在人类真菌病原体白色念珠菌中，环境pH对形态发生有深远的影响，并且对细胞外pH的响应与该真菌的致病性明显相关。酵母细胞已经进化出复杂的机制网络来响应环境pH，并且它们通常需要Rim 101和钙调神经磷酸酶/Crz 1信号通路的整合。Ca（2+）爆发是细胞在环境胁迫下的一种常见细胞反应;因此，在这项研究中，我们询问在碱性胁迫下是否也是如此，以及这种钙变化是否受Rim 101 p和Crz 1 p的调节。我们使用基于流式细胞术的方法证实了KOH刺激激活了钙内流，但在缺乏MID 1或CCH 1的细胞中，钙内流明显被取消。我们还发现，碱性pH诱导的PHO 89启动子的激活被阻断，没有相同的基因，而且，响应是Crz 1 p和Rim 101 p依赖。最后，我们研究了Rim 101 p和Crz 1 p通过MID 1、CCH 1和YVC 1基因对钙内流的调节作用，这些基因在rim 101 Δ/Δ和crz 1 Δ/Δ突变体中均下调。Ca 2+内流在碱胁迫反应中的重要作用及其调控机制提示Rim 101和Crz 1信号通路在C.白色念珠菌。
In the human fungal pathogen Candida albicans, environmental pH has profound effects on morphogenesis and response to extracellular pH is clearly relevant to the pathogenicity of this fungus. Yeast cells have evolved a complex network of mechanisms in response to the environmental pH and they often require the integration of the Rim101 and calcineurin/Crz1 signaling pathways. Ca(2+) burst is a common cellular response when cells are exposed to environmental stresses; therefore, in this study, we asked whether it follows the same case under alkaline stress and whether this calcium change is regulated by Rim101p and Crz1p. We confirmed the calcium influx was activated by KOH stimuli using a flow cytometry-based method, but it was obviously abolished in cells lacking MID1 or CCH1. We also found that alkaline pH-induced activation of the PHO89 promoter was blocked without the same gene; moreover, the response was Crz1p- and Rim101p-dependent. Finally, we investigated the regulation role of Rim101p and Crz1p in calcium influx through MID1, CCH1 and YVC1 genes, which were all downregulated in rim101Δ/Δ and crz1Δ/Δ mutants. The important role of calcium influx in the alkaline stress response and its regulation suggested a potential integration effect of Rim101 and Crz1 signaling pathways in C. albicans.