Kinetic and hydrodynamic properties of transducin: comparison of physical and structural parameters for GTP-binding regulatory proteins.
Kinetic and hydrodynamic properties of transducin: comparison of physical and structural parameters for GTP-binding regulatory proteins.
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转导蛋白的动力学和流体动力学特性：GTP 结合调节蛋白的物理和结构参数的比较。
DOI：
10.1021/bi00388a020
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发表时间：
1987
期刊：
影响因子：
2.9
通讯作者：
Johnson,GL
中科院分区：
文献类型：
--
作者：
Wessling-Resnick,M;Johnson,GL
材料与方法按照先前描述的方法（Wessling-Resnick &约翰逊，1987）从暗适应牛视网膜中分离转导蛋白。将视网膜在冰冷的20 mM Tris（pH 7.4）、1 mM CaCl 2和45%（w/w）蔗糖中照射并通过注射器数次破坏。通过浮选收集杆外节（ROS），在不含蔗糖的缓冲液中洗涤，并分层
Materials and MethodsPreparative Methods. Transducin was isolated from dark-adapted bovine retinas following procedures previously described (Wessling-Resnick & Johnson, 1987). Retinas were illuminated and disrupted in ice-cold 20 mM Tris, pH 7.4, 1 mM CaCl2, and 45%(w/w) sucrose by several passages through a syringe. Rod outer segments (ROS) were collected by flotation, washed in buffer without sucrose, and layered