Lactobacillus plantarum CBT LP3 ameliorates colitis via modulating T cells in mice
Lactobacillus plantarum CBT LP3 ameliorates colitis via modulating T cells in mice
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DOI：
10.1016/j.ijmm.2020.151391
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发表时间：
2020-02-01
影响因子：
4.1
通讯作者：
Cheon, Jae Hee
中科院分区：
文献类型：
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作者：
Kim, Da Hye;Kim, Soochan;Cheon, Jae Hee
植物乳杆菌由于其在免疫调节和维持肠道通透性中的作用而被鉴定为益生菌。本研究旨在探讨L. plantarum CBT LP3（LP3）。使用葡聚糖硫酸钠（DSS）在小鼠中诱导结肠炎进行该体内研究。将小鼠随机分为三组：对照组，供应正常饮用水; DSS处理组，随后口服给药载体; DSS处理组，在DSS给药后每天灌胃LP 3，持续7天。使用流式细胞术分析从腹膜腔细胞和脾细胞收获的巨噬细胞和T细胞亚群。采用定量逆转录聚合酶链反应测定基因表达和细胞因子谱。与对照相比，给予LP 3显著减弱疾病活动和组织病理学。LP 3具有抗炎作用，脾细胞中调节性T细胞和2型辅助性T细胞的诱导增加，杯状细胞的恢复伴随着促炎细胞因子表达的抑制。这些结果表明L.植物CBT LP 3可以用作有效的免疫调节剂，这对IBD治疗具有重要意义。
Lactobacillus plantarum has been identified as a probiotic bacterium owing to its role in immune regulation and maintenance of intestinal permeability. Here, we investigated the anti-colitic effects and mechanism of L. plantarum CBT LP3 (LP3). This in vivo study was performed using dextran sodium sulfate (DSS) to induce colitis in mice. Mice were randomly divided into three groups: a control supplied with normal drinking water, a DSS-treated group followed by oral administration of vehicle, and a DSS-treated group gavaged with LP3 daily for 7 days following DSS administration. An analysis of macrophages and T cell subsets harvesting from peritonium cavity cells and splenocytes was performed using a flow cytometric assay. Gene expression and cytokine profiles were measured using quantitative reverse transcriptase polymerase chain reaction. The administration of LP3 significantly attenuated disease activity and histolopathology compared to control. LP3 had anti-inflammatory effects, with increased induction of regulatory T cells and type 2 helper T cells in splenocytes and restoration of goblet cells accompanied by suppression of proinflammatory cytokine expressions. These findings suggest that L. plantarum CBT LP3 can be used as a potent immunomodulator, which has significant implications for IBD treatment.