Indian hedgehog contributes to human cartilage endplate degeneration
Indian hedgehog contributes to human cartilage endplate degeneration
复制标题
印度刺猬导致人类软骨终板退化
DOI：
10.1007/s00586-015-4000-4
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发表时间：
2015-08-01
影响因子：
2.8
通讯作者：
Wei, Lei
中科院分区：
文献类型：
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作者：
Wang, Shaowei;Yang, Kun;Wei, Lei
目的探讨印度刺猬（Indian hedgehog，Ihh）信号在人软骨终板（cartilage endplate，CEP）退变中的作用。方法收集9例Modic Ⅰ型和45例Modic Ⅱ型病变患者的CEP退变组织和17例椎体爆裂性骨折患者的正常组织。将标本切成薄片进行离体器官培养或消化以分离软骨细胞进行体外细胞培养。Ihh的表达和Ihh对软骨退变的影响，通过调查在本study.ResultsIhh的表达和软骨退变标志物显着增加的Modic I和II组。软骨退变标志物的表达与退变程度呈正相关。获得的功能Ihh促进表达的软骨退变标志物在体外，而失去的功能Ihh抑制其表达在体外和exvivo.Conclusions这些研究结果表明，Ihh促进CEP变性。阻断Ihh通路对减轻CEP变性具有潜在的临床应用价值。
PurposeTo determine the role of Indian hedgehog (Ihh) signaling in human cartilage endplate (CEP) degeneration.MethodsCEP-degenerated tissues from patients with Modic I or II changes (n= 9 and 45, respectively) and normal tissues from vertebral burst fracture patients (n= 17) were collected. Specimens were either cut into slices for organ culture ex vivo or digested to isolate chondrocytes for cell culture in vitro. Ihh expression and the effect of Ihh on cartilage degeneration were determined by investigating degeneration markers in this study.ResultsIhhexpression and cartilage degeneration markers significantly increased in the Modic I and II groups. The expression of cartilage degeneration markers was positively correlated with degeneration severity. Gain-of-function for Ihh promoted expression of cartilage degeneration markers in vitro, while loss-of-function for Ihh inhibited their expression both in vitro and ex vivo.ConclusionsThese findings demonstrated thatIhhpromotes CEP degeneration. Blocking Ihh pathway has potential clinical usage for attenuating CEP degeneration.