SACCHAROMYCES-BOULARDII INHIBITS CLOSTRIDIUM-DIFFICILE TOXIN-A BINDING AND ENTEROTOXICITY IN RAT ILEUM
SACCHAROMYCES-BOULARDII INHIBITS CLOSTRIDIUM-DIFFICILE TOXIN-A BINDING AND ENTEROTOXICITY IN RAT ILEUM
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DOI：
10.1016/0016-5085(93)90280-p
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发表时间：
1993-04-01
期刊：
影响因子：
29.4
通讯作者：
LAMONT, JT
中科院分区：
文献类型：
--
作者：
POTHOULAKIS, C;KELLY, CP;LAMONT, JT
背景资料：布拉酵母是一种非致病性酵母，用于预防和治疗艰难梭菌相关性腹泻和结肠炎。然而，S.方法：用S.在体外测量布拉氏培养的悬浮液或过滤的条件培养基。毒素A对大鼠回肠袢分泌、上皮通透性和形态学的影响也在S.结果：S.布拉地尔以剂量依赖性方式减少[3 H]毒素A-受体结合。回肠刷状缘十二烷基硫酸钠聚丙烯酰胺凝胶电泳。布拉第条件培养基显示所有刷状缘蛋白减少。用S.蛭形菌悬液可减少毒素A引起的体液分泌和甘露醇渗透性。布拉地尔可通过抑制毒素A-受体结合来降低毒素A的某些肠毒性作用。这种作用似乎是由酵母的分泌产物，可能是蛋白酶表现出来的。
Background: Saccharomyces boulardiiis a nonpathogenic yeast used for the prevention and treatment ofClostridium difficile-associated diarrhea and colitis. However, the mechanism by whichS. boulardiiexerts its protective effects remains unclear.Methods:The binding of [3H]toxin A to its brush border receptor preincubated withS. boulardii-cultured suspension or filtered conditioned medium was measured in vitro. The effect of toxin A on secretion, epithelial permeability, and morphology in rat ileal loops in vivo was also examined in rats pretreated withS. boulardii.Results: S. boulardiireduced [3H]toxin A-receptor binding in a dose-dependent fashion. Sodium dodecyl sulfate polyacrylamide gel electrophoresis of ileal brush border exposed toS. boulardii-conditioned medium revealed a diminution of all brush border proteins. Treatment of rats withS. boulardiisuspension reduced fluid secretion and mannitol permeability caused by toxin A.Conclusions: S. boulardiimay reduce some of the enterotoxic effects of toxin A by inhibiting toxin A-receptor binding. This effect appears to be manifested by a secreted product of the yeast, possibly a protease.