Osteoblast and bone tissue response to surface modified zirconia and titanium implant materials
Osteoblast and bone tissue response to surface modified zirconia and titanium implant materials
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DOI：
10.1016/j.dental.2013.04.003
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发表时间：
2013-07-01
期刊：
影响因子：
5
通讯作者：
Butz, Frank
中科院分区：
文献类型：
--
作者：
Kohal, Ralf J.;Baechle, Maria;Butz, Frank
目标。本研究考察了成骨细胞对一种新型酸蚀和喷砂氧化锆表面的体外和体内反应。成骨hFOB 1.19细胞分别在电化学阳极氧化钛（TiUnite (R)）、加工钛（Ti-m）、喷砂酸蚀氧化锆（TZP-proc）和加工氧化锆（TZP-A-m）上培养。利用三维激光扫描测量和扫描电镜分析了各种衬底的表面形貌。在培养第1、3、7、14、21和28天，测定细胞增殖情况。RT-PCR分析基因表达。对植入大鼠股骨的微型植入物进行了组织学分析和生物力学试验。在前7天，在TiUnite (R)表面观察到细胞增殖迟缓。培养28 d后，各表面细胞增殖水平基本一致。在第21天，可以看到tzp过程中骨和细胞外基质特异性基因的上调。愈合后14天和28天，TiUnite (R)的平均骨-种植体接触率分别高于TZP-proc。28天时，生物力学测试显示TiUnite (R)的数值显著高于其他所有表面。这种新颖、粗糙的氧化锆表面被hFOB 1.19细胞接受并整合到大鼠骨组织中。然而，与适度粗糙的钛表面相比，骨整合似乎进行得更慢，程度也更小。(C) 2013年牙科材料学会。Elsevier Ltd.出版。版权所有。
Objective. This study examined the in vitro and in vivo response of osteoblasts to a novel, acid-etched and sandblasted zirconia surface.Methods. Osteoblastic hFOB 1.19 cells were cultured either on electrochemically anodized titanium (TiUnite (R)), machined titanium (Ti-m), sandblasted and acid-etched zirconia (TZP-proc), and machined zirconia (TZP-A-m). The surface topography of the various substrates was analyzed by 3D laserscan measurements and scanning electron microscopy. At culture days 1, 3, 7, 14, 21, and 28, cell proliferation was determined. Gene expression was analyzed using RT-PCR. Histologic analysis and biomechanical testing was performed on miniature implants placed in the rat femur.Results. During the first 7 days, a retarded cell proliferation was observed on the TiUnite (R) surface. After 28 days of cultivation, cell proliferation reached similar levels on all surfaces. An up-regulation of bone and extracellular matrix specific genes could be seen for TZP-proc at day 21. The mean bone-implant contact rate after a healing period of 14 and 28 days, respectively, was higher for TiUnite (R) than for TZP-proc. At 28 day, the biomechanical test showed significantly higher values for TiUnite (R) than for all other surfaces.Significance. The novel, rough zirconia surface was accepted by hFOB 1.19 cells and integrates into rat bone tissue. However, osseointegration seemed to proceed more slowly and to a lesser extent compared to a moderately roughened titanium surface. (C) 2013 Academy of Dental Materials. Published by Elsevier Ltd. All rights reserved.