Extracellular Vesicles From the Cotton Pathogen Fusarium oxysporum f. sp. vasinfectum Induce a Phytotoxic Response in Plants
Extracellular Vesicles From the Cotton Pathogen Fusarium oxysporum f. sp. vasinfectum Induce a Phytotoxic Response in Plants
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DOI：
10.3389/fpls.2019.01610
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发表时间：
2020-01-10
影响因子：
5.6
通讯作者：
Anderson, Marilyn A.
中科院分区：
文献类型：
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作者：
Bleackley, Mark;Samuel, Monisha;Anderson, Marilyn A.
细胞外囊泡（EV）代表了协调分泌多种分子货物（包括蛋白质、脂质、核酸和代谢物）的系统。它们在多细胞生物的细胞间通讯中起着重要作用，最近被认为与宿主-病原体相互作用有关。EV在真菌生物学中的作用的研究集中在致病性酵母，这是人类的主要病原体。在这项研究中，我们扩大了调查真菌EV植物病原体，特别是主要的棉花病原体尖孢镰刀菌f。vasinfectum.从F. oxysporum F. vasinfectum培养基具有类似于来自酵母如白色念珠菌和新型隐球菌EV的形态和大小分布。F.电动汽车的独特功能。oxysporum F. sp. vasinfectum是它们的紫色，这被预测是由被包装到EV中的萘醌色素引起的。蛋白质组学分析。oxysporum F. vasinfectum EV的研究表明，它们富含在聚酮化合物合成中起作用的蛋白质以及在基本细胞过程中起作用的蛋白酶和蛋白质。F. oxysporum F. sp. vasinfectum EV侵染棉花或N. benthamiana植物导致植物毒性反应。这些观察导致了一个假设，即F。oxysporum F. sp. vasinfectum EV可能在感染过程中发挥关键作用。
Extracellular vesicles (EVs) represent a system for the coordinated secretion of a variety of molecular cargo including proteins, lipids, nucleic acids, and metabolites. They have an essential role in intercellular communication in multicellular organisms and have more recently been implicated in host-pathogen interactions. Study of the role for EVs in fungal biology has focused on pathogenic yeasts that are major pathogens in humans. In this study we have expanded the investigation of fungal EVs to plant pathogens, specifically the major cotton pathogen Fusarium oxysporum f. sp. vasinfectum. EVs isolated from F. oxysporum f. sp. vasinfectum culture medium have a morphology and size distribution similar to EVs from yeasts such as Candida albicans and Cryptococcus neoformans. A unique feature of the EVs from F. oxysporum f. sp. vasinfectum is their purple color, which is predicted to arise from a napthoquinone pigment being packaged into the EVs. Proteomic analysis of F. oxysporum f. sp. vasinfectum EVs revealed that they are enriched in proteins that function in synthesis of polyketides as well as proteases and proteins that function in basic cellular processes. Infiltration of F. oxysporum f. sp. vasinfectum EVs into the leaves of cotton or N. benthamiana plants led to a phytotoxic response. These observations lead to the hypothesis that F. oxysporum f. sp. vasinfectum EVs are likely to play a crucial role in the infection process.