NADPH dependent superoxide generation in the ovary and uterus of mice during estrous cycle and early pregnancy
NADPH dependent superoxide generation in the ovary and uterus of mice during estrous cycle and early pregnancy
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DOI：
10.1016/s0024-3205(00)00417-3
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发表时间：
2000-02-11
期刊：
影响因子：
6.1
通讯作者：
Laloraya, M
中科院分区：
文献类型：
--
作者：
Jain, S;Saxena, D;Laloraya, M
NADPH依赖性O-2（.-）使用超氧化物捕获自旋阱的时间推移电子自旋共振增强的新技术来估计周期小鼠和怀孕小鼠的卵巢和子宫中的产生。NADPH依赖性O-2（.-）在周期性雌性动物的早期排卵前阶段和妊娠动物的延长黄体期，小鼠卵巢中的生殖增加。子宫NADPH依赖性O-2（.-）在妊娠动物的着床前阶段，周期动物在发情前期的生产表明，这种酶在各个阶段对产生高水平的超氧阴离子自由基有贡献。卵巢和子宫NADPH依赖性O-2（.-）生产似乎是LH诱导的。
NADPH dependent O-2(.-) production in the ovary and uterus of cycling and pregnant mice was estimated employing a novel technique of time-lapse electron spin resonance enhancement of a superoxide-trapping spin trap. The NADPH dependent O-2(.-) generation in the mouse ovary increased during the early pre-ovulatory phase in cycling females and during extended luteal phase in pregnant animals. A peak of uterine NADPH dependent O-2(.-) production at proestrous in the cycling animals at pre-implantation phase in pregnant animals suggested a contribution of this enzyme towards generating high levels of superoxide anion radical during the respective stages. Both ovarian and uterine NADPH dependent O-2(.-) production appeared to be LH-inducible.