Zirconia substrate with periodic surface microstructures enhances osteogenic differentiation of rat adipose-derived stem cells
Zirconia substrate with periodic surface microstructures enhances osteogenic differentiation of rat adipose-derived stem cells
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具有周期性表面微结构的氧化锆基质增强大鼠脂肪干细胞的成骨分化
DOI：
10.1016/j.matlet.2022.133544
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发表时间：
2023
影响因子：
3
通讯作者：
Ito Atsuo
中科院分区：
文献类型：
--
作者：
Yasunaga Mayu;Watanabe Tomoko;Yano Gen;Murotomi Kazutoshi;Hiramatsu Miki;Hirose Motohiro;Kakehata Masayuki;Yashiro Hidehiko;Yamazaki Atsushi;Ito Atsuo
飞秒激光照射形成的具有周期性表面微结构的氧化锆衬底先前促进了大鼠骨髓间充质干细胞(BMSCs)的成骨分化，并伴随着细胞附着面积的减少。脂肪来源的间充质干细胞(ADSC)在临床上比骨髓间充质干细胞(BMSCs)更容易获得细胞。在本研究中，我们研究了具有周期性表面微结构的氧化锆基质对大鼠脂肪干细胞成骨分化的影响。具有周期性表面微结构的氧化锆基质不仅促进了大鼠ADSCs的成脂分化，而且促进了成骨分化，并伴随着细胞附着面积的减少。因此，在骨髓间充质干细胞无法获得的情况下，利用飞秒激光照射形成具有表面周期性微结构的氧化锆基质可用于ADSCs的骨组织工程。
Zirconia substrates with periodic surface microstructures formed by irradiation with a femtosecond laser previously enhanced osteogenic differentiation of rat bone marrow mesenchymal stem cells (BMSCs), associated with a reduction in the cell attachment area. Adipose-derived mesenchymal stem cells (ADSCs) are clinically less difficult in harvesting the cells than BMSCs. In this study, we investigated the effects of zirconia substrates with the periodic surface microstructures on osteogenic differentiation of rat ADSCs. Zirconia substrates with the periodic surface microstructures enhanced not adipogenic differentiation but osteogenic differentiation of rat ADSCs, associated with a reduction in the cell attachment area. Therefore, zirconia substrates with surface periodic microstructures formed by irradiation with a femtosecond laser could be useful in bone tissue engineering using ADSCs when BMSCs are unavailable.