Decapentaplegic signaling regulates Wingless ligand production and target activation during Drosophila wing development
Decapentaplegic signaling regulates Wingless ligand production and target activation during Drosophila wing development
复制标题
果蝇翅膀发育过程中十肢瘫痪信号调节无翅配体的产生和靶点激活
DOI：
10.1002/1873-3468.13713
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发表时间：
2020
期刊：
影响因子：
3.5
通讯作者：
Zhang Junzheng
中科院分区：
文献类型：
--
作者：
Li Yunlong;Zhang Fengchao;Jiang Na;Liu Tong;Shen Jie;Zhang Junzheng
十肢麻痹（Dpp）和无翅（Wg）信号通路对动物发育至关重要。然而，这两种信号是如何在不同的组织中整合的还不完全清楚。在这里，我们描述了一种新的模式Dpp-Wg串扰在果蝇翅膀的发展。我们表明，典型的Dpp信号是Wg靶基因激活所必需的。此外，Dpp信号通过schnurri（shn）阻遏物复合物抑制wg的转录。在wg基因的基因组位点中鉴定了Dpp响应性shn/pMad/Med沉默元件（SSE）。ChIP分析表明，SHN在体内与该元件相互作用。我们的研究结果支持一个模型，其中Dpp信号在wg基因和下游靶点的转录调控中起着双重作用。
The Decapentaplegic (Dpp) and Wingless (Wg) signaling pathways are essential for animal development. However, how these two signals are integrated in distinct tissues is not fully understood. Here, we describe a novel mode of Dpp-Wg crosstalk during Drosophila wing development. We show that the canonical Dpp signaling is required for Wg target gene activation. In addition, Dpp signaling inhibits the transcription of wg through the schnurri (shn) repressor complex. A Dpp-responsive shn/pMad/Med silencer element (SSE) is identified in the genomic loci of the wg gene. ChIP analysis suggests that shn interacts with this element in vivo. Our findings support a model in which Dpp signaling plays a dual role in transcriptional regulation of both the wg gene and downstream targets.