Isolation of tissue mast cells.
Isolation of tissue mast cells.
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DOI：
10.1002/0471142735.im0725s90
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发表时间：
2010-08-01
影响因子：
--
通讯作者：
Metcalfe, Dean D
中科院分区：
文献类型：
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作者：
Kulka, Marianna;Metcalfe, Dean D
肥大细胞主要存在于组织中，是过敏性炎症的主要效应细胞之一。肥大细胞来源于单核前体细胞，它们在组织中经历了最后的分化阶段。肥大细胞表达一组独特的蛋白酶，并根据它们分化的组织表现出功能多样性——这种现象通常被称为肥大细胞异质性。酶消化和密度离心通常用于从肺和皮肤等组织中分离人类肥大细胞，通常导致细胞活力和纯度低。在这里，我们描述了一个方案，结合温和的酶消化和积极的选择技术，以分离合理可行的和实质性丰富的组织肥大细胞的制剂。
Located primarily in tissues, mast cells are one of the principal effector cells in allergic inflammation. Mast cells derive from mononuclear precursor cells which undergo their final phase of differentiation in the tissues. Mast cells express a unique set of proteases and display functional diversity depending on the tissue in which they differentiate--a phenomenon often referred to as mast cell heterogeneity. Enzymatic digestion and density centrifugation have often been used to isolate human mast cells from tissues such as lung and skin, frequently resulting in cells with low viability and purity. Here, we describe a protocol that combines gentle enzymatic digestion with positive selection techniques to isolate reasonably viable and substantially enriched preparations of tissue mast cells.