Simultaneously sensitive detection of multiple miRNAs based on a strand displacement amplification
Simultaneously sensitive detection of multiple miRNAs based on a strand displacement amplification
复制标题
基于链置换扩增同时灵敏地检测多个 miRNA
DOI：
10.1039/c2cc36728a
复制
发表时间：
2013-01-01
影响因子：
4.9
通讯作者：
Zhou, Xiang
中科院分区：
文献类型：
--
作者：
Tian, Tian;Xiao, Heng;Zhou, Xiang
我们报道了一种基于链置换扩增的血清miRNA检测方法。在这样的系统中，通过荧光标记探针、共同引物和聚合酶的组合，可以实现对miRNA的多重、灵敏和快速检测。这可能潜在地用于临床领域，以实现疾病的早期诊断和预后。
We reported an efficient strategy based on a strand displacement amplification for serum miRNA detection. In such a system, a multiplexed, sensitive and quick detection of miRNAs could be achieved through a combination of fluorescence labeled probes, a common primer and a polymerase. This could be potentially used in the clinical field to achieve early disease diagnosis and prognosis.