Sequencing and comparative genomics analysis of the IncHI2 plasmids pT5282-mphA and p112298-catA and the IncHI5 plasmid pYNKP001-dfrA
Sequencing and comparative genomics analysis of the IncHI2 plasmids pT5282-mphA and p112298-catA and the IncHI5 plasmid pYNKP001-dfrA
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DOI：
10.1016/j.ijantimicag.2017.01.021
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发表时间：
2017-06-01
影响因子：
10.8
通讯作者：
Zhou, Dongsheng
中科院分区：
文献类型：
--
作者：
Liang, Quanhui;Yin, Zhe;Zhou, Dongsheng
IncHI质粒是肠杆菌科细菌耐药的重要载体。本研究基于测序的IncHI质粒，提出了将IncHI分型为5个独立聚类亚群的方案，包括先前指定的IncHI1-3和IncHI4-5。测定临床分离的阴沟肠杆菌、弗氏Citrobacter freundii和禽毒拉乌氏菌分离株的IncHI2质粒pT5282-mphA和p112298-catA以及IncHI5质粒pYNKP001-dfrA的全核苷酸序列，并与IncHI2和IncHI5参考质粒进行比较。IncHI2亚群和IncHI5亚群的主链序列具有一定的遗传保守性，但两个亚群的主链序列存在显著差异。然而，在这5个质粒中均存在偶转移区tra1和tra2以及抗碲基因簇ter。许多与整合子、转座子和基于插入序列的可移动元件相关的辅助区域被插入到质粒主干的不同位置，其中有几个大区域含有对多种抗生素具有抗性的基因。本研究产生的数据使我们对inchi型抗性质粒的多样性有了更深入的了解。(C) 2017 Elsevier B.V.和国际化学疗法学会。版权所有。
Incompatibility group IncHI plasmids are important vectors of antibiotic resistance in Enterobacteriaceae. In this study, a scheme for typing IncHI into five separately clustering subgroups, including previously designated IncHI1-3 as well as IncHI4-5, was proposed based on sequenced IncHI plasmids. The complete nucleotide sequences of the IncHI2 plasmids pT5282-mphA and p112298-catA and the IncHI5 plasmid pYNKP001-dfrA from clinical Enterobacter cloacae, Citrobacter freundii and Raoultella ornithinolytica isolates, respectively, were determined and were compared with IncHI2 and IncHI5 reference plasmids. Considerable genetic conservation was observed within the backbone sequences of each of the IncHI2 and IncHI5 subgroups, but the backbone sequences of the two subgroups were dramatically different from each other. However, the conjugal transfer regions tra1 and tra2 as well as the tellurium resistance gene cluster ter were present in all five plasmids. A number of accessory regions associated with integrons, transposons and insertion sequence-based mobile elements have been inserted at various sites of the plasmid backbones, among which were several large regions harbouring genes conferring resistance to multiple classes of antibiotics. Data generated from this study provide us with a deeper understanding of the diversification of IncHI-type resistance plasmids. (C) 2017 Elsevier B.V. and International Society of Chemotherapy. All rights reserved.