Estimating the size and number of autophagic bodies by electron microscopy
Estimating the size and number of autophagic bodies by electron microscopy
复制标题
通过电子显微镜估计自噬体的大小和数量
DOI：
10.4161/auto.26856
复制
发表时间：
2014-01-01
期刊：
影响因子：
13.3
通讯作者：
Klionsky, Daniel J.
中科院分区：
文献类型：
--
作者：
Backues, Steven K.;Chen, Dachuan;Klionsky, Daniel J.
Much recent and ongoing research is focused on understanding the mechanisms and regulation of autophagy, a cellular self-degradation pathway with many links to human health. Although many assays exist to measure the total magnitude of autophagy, electron microscopy remains the tool of choice for the determination of the size and the number of autophagosomes formed in a given mutant or under given induction conditions. Here we present a detailed protocol for measuring autophagic bodies in the yeast Saccharomyces cerevisiae by electron microscopy. Furthermore, we present an improved mathematical method for estimating body size and a new method for estimating body number. Finally, we include a discussion of the merits and limitations of these methods and an example of their application to autophagic bodies formed in the ume6 strain.
