An InDel in the Promoter of Al-ACTIVATED MALATE TRANSPORTER9 Selected during Tomato Domestication Determines Fruit Malate Contents and Aluminum Tolerance
An InDel in the Promoter of Al-ACTIVATED MALATE TRANSPORTER9 Selected during Tomato Domestication Determines Fruit Malate Contents and Aluminum Tolerance
复制标题
番茄驯化过程中选择的铝激活苹果酸转运蛋白9启动子中的插入缺失决定果实苹果酸含量和铝耐受性
DOI：
10.1105/tpc.17.00211
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发表时间：
2017-09-01
期刊：
影响因子：
11.6
通讯作者：
Ye, Zhibiao
中科院分区：
文献类型：
--
作者：
Ye, Jie;Wang, Xin;Ye, Zhibiao
破译植物中苹果酸积累的机制将有助于更好地理解植物化学，这对改善作物品种的风味质量和增强人类健康益处具有重要意义。然而，苹果酸代谢的调节在作物如番茄（Solanum lycopersicum）中知之甚少。在这里，我们整合了一个基于代谢产物的全基因组关联研究与连锁图谱和基因功能研究，以表征苹果酸积累的遗传学在全球收集的番茄种质资源具有广泛的遗传多样性。我们报告，TFM 6（番茄果实苹果酸6），这对应于铝激活的苹果酸转运蛋白9（S1-ALMT 9在番茄），是主要的数量性状基因座负责水果苹果酸积累的变异番茄基因型。在S1-ALMT 9的启动子区域中的3-bp插入缺失与高果实苹果酸含量相关联。进一步的分析表明，该插入缺失破坏了S1-ALMT 9启动子中的W盒结合位点，其阻止WRKY转录阻遏物S1-WRKY 42的结合，从而减轻了S1-ALMT 9表达的阻遏并促进了高果实苹果酸积累。进化分析表明，这种高表达的Sl-ALMT 9等位基因在番茄驯化过程中被选择。此外，液泡膜定位的S1-ALMT 9增加的丰度铝处理后，从而提高苹果酸运输和增强铝抗性。
Deciphering the mechanism of malate accumulation in plants would contribute to a greater understanding of plant chemistry, which has implications for improving flavor quality in crop species and enhancing human health benefits. However, the regulation of malate metabolism is poorly understood in crops such as tomato (Solanum lycopersicum). Here, we integrated a metabolite-based genome-wide association study with linkage mapping and gene functional studies to characterize the genetics of malate accumulation in a global collection of tomato accessions with broad genetic diversity. We report that TFM6 (tomato fruit malate 6), which corresponds to Al-ACTIVATED MALATE TRANSPORTER9 (Sl-ALMT9 in tomato), is the major quantitative trait locus responsible for variation in fruit malate accumulation among tomato genotypes. A 3-bp indel in the promoter region of Sl-ALMT9 was linked to high fruit malate content. Further analysis indicated that this indel disrupts a W-box binding site in the Sl-ALMT9 promoter, which prevents binding of the WRKY transcription repressor Sl-WRKY42, thereby alleviating the repression of Sl-ALMT9 expression and promoting high fruit malate accumulation. Evolutionary analysis revealed that this highly expressed Sl-ALMT9 allele was selected for during tomato domestication. Furthermore, vacuole membrane-localized Sl-ALMT9 increases in abundance following Al treatment, thereby elevating malate transport and enhancing Al resistance.