Improved drought and salt tolerance of Arabidopsis thaliana by transgenic expression of a novel DREB gene from Leymus chinensis
Improved drought and salt tolerance of Arabidopsis thaliana by transgenic expression of a novel DREB gene from Leymus chinensis
复制标题
通过转基因表达羊草中的新型 DREB 基因提高拟南芥的耐旱性和耐盐性
DOI：
10.1007/s00299-011-1058-2
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发表时间：
2011-08-01
影响因子：
6.2
通讯作者：
Liu Gongshe
中科院分区：
文献类型：
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作者：
Peng Xianjun;Ma Xingyong;Liu Gongshe
脱水反应元件结合蛋白（DREB）是植物逆境应答和信号转导中的重要转录因子。基于高通量测序结果，利用RACE PCR技术从抗旱牧草羊草中克隆到一个新的LcDREB 3a转录因子cDNA序列。序列相似性分析表明，该基因产物在ABA反应途径中是活跃的，基于实时PCR的表达分析显示，转录本在各种应激处理中积累。这些结果表明，LcDREB3a在L.中国。作为DREB转录因子的基因产物的身份是支持其核定位的观察时，在洋葱表皮细胞中瞬时表达为GFP融合。此外，LcDREB3a能够激活报告基因表达，并且在酵母单杂交测定中显示该蛋白质特异性结合保守的DRE元件。与对照相比，LcDREB 3a在拟南芥中的转基因表达不会导致生长迟缓，并诱导胁迫耐受基因的表达增加，从而提高干旱和盐胁迫的耐受性。因此，LcDREB3a编码一个逆境诱导DREB转录因子，能够增强植物的非生物逆境耐性。
Dehydration-responsive element-binding (DREB) proteins are important transcription factors in plant stress responses and signal transduction. Based on high-throughput sequencing results, a new cDNA sequence encoding an LcDREB3a transcription factor from the drought-resistant forage grass, Leymus chinensis, was isolated by RACE PCR. Sequence similarity analysis indicates that the gene product is active in the ABA-responsive pathway, and real-time PCR-based expression analysis shows the transcript accumulates in response to a variety of stress treatments. These results indicate that LcDREB3a is involved in both ABA-dependent and -independent signal transduction in the stress-responsive process of L. chinensis. The identity of the gene product as a DREB transcription factor is supported by observations of its nuclear localization when transiently expressed as a GFP fusion in onion epidermal cells. Furthermore, LcDREB3a is able to activate reporter gene expression, and the protein is shown to specifically bind to the conserved DRE element in a yeast one-hybrid assay. The transgenic expression of LcDREB3a in Arabidopsis causes no growth retardation and induces the increased expression of stress tolerance genes compared to control, resulting in improved drought and salt stress tolerance. Thus, LcDREB3a, encoding a stress-inducible DREB transcription factor, could enhance the abiotic stress tolerance of plants.