Expression of progesterone receptor isoforms in corpora lutea of human subjects: correlation with serum oestrogen and progesterone concentrations
Expression of progesterone receptor isoforms in corpora lutea of human subjects: correlation with serum oestrogen and progesterone concentrations
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DOI：
10.1093/molehr/4.11.1045
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发表时间：
1998-11-01
影响因子：
4
通讯作者：
Tamaya, T
中科院分区：
文献类型：
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作者：
Misao, R;Nakanishi, Y;Tamaya, T
为探讨孕酮受体A型（PR-A）和B型（PR-B）在人黄体中的生物学意义，本研究同时测定了人黄体中PR-A和PR-B mRNA的表达和血清类固醇激素浓度。在所有分析的样品中，PR-A mRNA的表达均高于PR-B mRNA。总PR（PR-AB）和PR-B mRNA在月经周期分泌晚期显著低于分泌早期和中期（P < 0.01）。PR-B与PR-AR mRNA的比值在分泌期无明显变化。在分泌早期和中期，PR-B mRNA含量与血清孕酮含量呈负相关，PR-B/PR-AB mRNA比值与血清孕酮含量呈负相关（P < 0.01）。这些结果表明，人黄体可能在细胞内合成PR-A和PR-B，从而参与了黄体的类固醇功能调节，尤其是在分泌的早期和中期，孕酮可能调节PR-A和PR-B的合成。
To understand the biological significance of progesterone receptor forms A (PR-A) and B (PR-B) in human corpus luteum, the expression of mRNA and serum steroid hormone concentrations were determined simultaneously in the luteal stages. The expression of PR-A mRNA predominated over PR-B mRNA in all samples analysed. Total PR (PR-AB) and PR-B mRNA concentrations at the late secretory phase were significantly (P < 0.01) lower than those at the early and mid secretory phases of the menstrual cycle. The ratio of PR-B to PR-AR mRNA concentration showed no significant change during the secretory phase. In the early and mid secretory phases, there was a negative correlation between PR-B mRNA concentration and serum progesterone concentration, and between the ratio of PR-B to PR-AB mRNA concentrations and serum progesterone concentration (P < 0.01). These findings suggest that human corpus luteum might intracellularly synthesize PR-A and PR-B, and thus be involved in the steroid functional regulation of the corpus luteum, especially at the early and mid secretory phases, and that progesterone might regulate the synthesis of PR-A and PR-B.