Transcription factor expression patterns in bovine in vitro-derived embryos prior to maternal-zygotic transition
Transcription factor expression patterns in bovine in vitro-derived embryos prior to maternal-zygotic transition
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DOI：
10.1095/biolreprod.103.022970
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发表时间：
2004-06-01
影响因子：
3.6
通讯作者：
Sirard, MA
中科院分区：
文献类型：
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作者：
Vigneault, C;McGraw, S;Sirard, MA
母合子转换（MZT）是一个复杂的现象，其特征是在胚胎中启动转录并将母体mRNA替换为胚胎mRNA。为了实现这一点，转录激活需要各种因素和条件。我们的假设是，在牛前MZT胚胎中缺乏转录是由于，在一定程度上，一个不完整的或休眠的转录装置。因此，根据这一假设，功能性转录机制应该出现在八细胞牛胚胎中，以促进MZT期间的胚胎转录。考虑到这一点，我们研究了在植入前胚胎发育过程中所选择的转录因子的存在，以确定它们的转录水平如何在牛前MZT胚胎中变化。为了实现这一目标，实时逆转录-聚合酶链反应被用来定量几种不同转录因子的mRNA水平（YY 1、HMGA 1、RY-1、P300、CREB、YAP 65、HMGN 1、HMGB 1、NFAR、OCT-4、TEAD 2、ATF-1、HMGN 2、MSY 2和TBP）在牛生殖囊泡（GV）和中期II（MII）卵母细胞中以及在二细胞、四细胞、八细胞、和体外产生的胚泡期胚胎。我们的研究结果表明，所有的基因研究可以分为五个不同的类别，根据其mRNA表达模式在发育阶段观察。总之，研究的所有转录因子均存在于MZT前胚胎中，其中许多转录因子的表达模式表明在MZT中具有潜在作用。
Maternal-zygotic transition (MZT) is a complex phenomenon characterized by the initiation of transcription in the embryo and the replacement of maternal mRNA with embryonic mRNA. In order for this to occur, transcriptional activation requires various factors and conditions. Our hypothesis is that the lack of transcription in the bovine pre-MZT embryo is due, in part, to an incomplete or dormant transcriptional apparatus. Therefore, in accordance with this hypothesis, functioning transcriptional mechanisms should appear in the eight-cell bovine embryo to facilitate embryonic transcription during the MZT. With this in mind, we examined the presence of selected transcription factors during preimplantation embryo development to establish how their transcript levels change in bovine pre-MZT embryos. To achieve this goal, real-time reverse transcription-polymerase chain reaction was used to quantify the mRNA level of several different transcription factors (YY1, HMGA1, RY-1, P300, CREB, YAP65, HMGN1, HMGB1, NFAR, OCT-4, TEAD2, ATF-1, HMGN2, MSY2, and TBP) in germinal vesicle (GV) and metaphase II (MII) bovine oocytes and in two-, four-, eight-cell, and blastocyst stage embryos produced in vitro. Our results demonstrate that all genes examined can be grouped into five different categories according to their mRNA expression patterns at the developmental stages observed. To summarize, all transcription factors studied were present in pre-MZT embryos and the expression pattern of many of them suggest a potential role in MZT.